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Regional collagen turnover and composition of the human patellar tendon

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@article{d8b9c3ba58f54559b3d929c913ba520b,
title = "Regional collagen turnover and composition of the human patellar tendon",
abstract = "Tendon pathology (tendinopathy) typically occurs in specific regions of a tendon, and growth in response to exercise also appears to be more pronounced in specific regions. In a previous study in animals we found evidence of regional differences in tendon turnover, but whether the turnover of human patellar tendon differs in different regions still remains unknown. Patellar tendons were obtained from cadavers of healthy men and women (body donation program, n = 5 donors, >60 yr of age). Samples were taken from 10 different regions along the length, width, and thickness of the tendon. Turnover was measured by 14C bomb pulse dating and also estimated from the accumulation of advanced glycation end products (AGEs) by fluorescence (340/460 nm) in addition to measurement of specific AGEs by mass spectrometry. Composition in terms of collagen, glycosaminoglycans (GAGs), and DNA was also assessed in each region. 14C results showed that all tendon regions had a similar 14C concentration, which was equal to the average atmospheric 14C concentration during the first 15 yr of the person's life. Fluorescence normalized to dry weight did not differ between regions, nor did specific AGEs. Higher GAG content was observed in the proximal and near the distal insertion of the tendon. In conclusion, healthy human patellar tendon displays no regional differences in collagen turnover throughout life. ",
author = "Cheng Zhang and Christian Coupp{\'e} and Scheijen, {Jean L J M} and Schalkwijk, {Casper G} and Michael Kjaer and Magnusson, {S Peter} and Svensson, {Rene B}",
year = "2020",
month = apr,
day = "1",
doi = "10.1152/japplphysiol.00030.2020",
language = "English",
volume = "128",
pages = "884--891",
journal = "Journal of Applied Physiology",
issn = "0161-7567",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Regional collagen turnover and composition of the human patellar tendon

AU - Zhang, Cheng

AU - Couppé, Christian

AU - Scheijen, Jean L J M

AU - Schalkwijk, Casper G

AU - Kjaer, Michael

AU - Magnusson, S Peter

AU - Svensson, Rene B

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Tendon pathology (tendinopathy) typically occurs in specific regions of a tendon, and growth in response to exercise also appears to be more pronounced in specific regions. In a previous study in animals we found evidence of regional differences in tendon turnover, but whether the turnover of human patellar tendon differs in different regions still remains unknown. Patellar tendons were obtained from cadavers of healthy men and women (body donation program, n = 5 donors, >60 yr of age). Samples were taken from 10 different regions along the length, width, and thickness of the tendon. Turnover was measured by 14C bomb pulse dating and also estimated from the accumulation of advanced glycation end products (AGEs) by fluorescence (340/460 nm) in addition to measurement of specific AGEs by mass spectrometry. Composition in terms of collagen, glycosaminoglycans (GAGs), and DNA was also assessed in each region. 14C results showed that all tendon regions had a similar 14C concentration, which was equal to the average atmospheric 14C concentration during the first 15 yr of the person's life. Fluorescence normalized to dry weight did not differ between regions, nor did specific AGEs. Higher GAG content was observed in the proximal and near the distal insertion of the tendon. In conclusion, healthy human patellar tendon displays no regional differences in collagen turnover throughout life.

AB - Tendon pathology (tendinopathy) typically occurs in specific regions of a tendon, and growth in response to exercise also appears to be more pronounced in specific regions. In a previous study in animals we found evidence of regional differences in tendon turnover, but whether the turnover of human patellar tendon differs in different regions still remains unknown. Patellar tendons were obtained from cadavers of healthy men and women (body donation program, n = 5 donors, >60 yr of age). Samples were taken from 10 different regions along the length, width, and thickness of the tendon. Turnover was measured by 14C bomb pulse dating and also estimated from the accumulation of advanced glycation end products (AGEs) by fluorescence (340/460 nm) in addition to measurement of specific AGEs by mass spectrometry. Composition in terms of collagen, glycosaminoglycans (GAGs), and DNA was also assessed in each region. 14C results showed that all tendon regions had a similar 14C concentration, which was equal to the average atmospheric 14C concentration during the first 15 yr of the person's life. Fluorescence normalized to dry weight did not differ between regions, nor did specific AGEs. Higher GAG content was observed in the proximal and near the distal insertion of the tendon. In conclusion, healthy human patellar tendon displays no regional differences in collagen turnover throughout life.

UR - http://www.scopus.com/inward/record.url?scp=85083621755&partnerID=8YFLogxK

U2 - 10.1152/japplphysiol.00030.2020

DO - 10.1152/japplphysiol.00030.2020

M3 - Journal article

C2 - 32163333

VL - 128

SP - 884

EP - 891

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 0161-7567

IS - 4

ER -

ID: 61626691