TY - JOUR
T1 - Rapid increase of genetically diverse methicillin-resistant Staphylococcus aureus, Copenhagen, Denmark
AU - Bartels, Mette Damkjaer
AU - Boye, Kit
AU - Rhod Larsen, Anders
AU - Skov, Robert
AU - Westh, Henrik
PY - 2007
Y1 - 2007
N2 - In Copenhagen, methicillin-resistant Staphylococcus aureus (MRSA) accounted for <15 isolates per year during 1980-2002. However, since 2003 an epidemic increase has been observed, with 33 MRSA cases in 2003 and 110 in 2004. We analyzed these 143 cases epidemiologically and characterized isolates by pulsed-field gel electrophoresis, Staphylococcus protein A (spa) typing, multilocus sequence typing, staphylococcal chromosome cassette (SCC) mec typing, and detection of Panton-Valentine leukocidin (PVL) genes. Seventy-one percent of cases were community-onset MRSA (CO-MRSA); of these, 36% had no identified risk factors. We identified 29 spa types (t) and 16 sequence types (STs) belonging to 8 clonal complexes and 3 ST singletons. The most common clonal types were t024/ST8-IV, t019/ST30-IV, t044/ST80-IV, and t008/ST8-IV (USA300). A total of 86% of isolates harbored SCCmec IV, and 44% had PVL. Skin and soft tissue infections dominated. CO-MRSA with diverse genetic backgrounds is rapidly emerging in a low MRSA prevalence area.
AB - In Copenhagen, methicillin-resistant Staphylococcus aureus (MRSA) accounted for <15 isolates per year during 1980-2002. However, since 2003 an epidemic increase has been observed, with 33 MRSA cases in 2003 and 110 in 2004. We analyzed these 143 cases epidemiologically and characterized isolates by pulsed-field gel electrophoresis, Staphylococcus protein A (spa) typing, multilocus sequence typing, staphylococcal chromosome cassette (SCC) mec typing, and detection of Panton-Valentine leukocidin (PVL) genes. Seventy-one percent of cases were community-onset MRSA (CO-MRSA); of these, 36% had no identified risk factors. We identified 29 spa types (t) and 16 sequence types (STs) belonging to 8 clonal complexes and 3 ST singletons. The most common clonal types were t024/ST8-IV, t019/ST30-IV, t044/ST80-IV, and t008/ST8-IV (USA300). A total of 86% of isolates harbored SCCmec IV, and 44% had PVL. Skin and soft tissue infections dominated. CO-MRSA with diverse genetic backgrounds is rapidly emerging in a low MRSA prevalence area.
KW - Communicable Diseases, Emerging
KW - Community-Acquired Infections
KW - Cross Infection
KW - Denmark
KW - Evolution, Molecular
KW - Genetic Variation
KW - Humans
KW - Methicillin Resistance
KW - Phylogeny
KW - Staphylococcal Infections
KW - Staphylococcus aureus
M3 - Journal article
C2 - 18258003
SN - 1080-6040
VL - 13
SP - 1533
EP - 1540
JO - Emerging Infectious Diseases (Print Edition)
JF - Emerging Infectious Diseases (Print Edition)
IS - 10
ER -