Rapid in vitro and in vivo Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria

Artur Schmidtchen, Manoj Puthia

Abstract

Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are time-consuming and do not allow for longitudinal analysis. Bioluminescence imaging is sensitive and gives vital spatial and temporal information on the infection status in the body. Here, using bioluminescent Pseudomonas aeruginosa, we describe an in vitro and an in vivo approach to rapidly evaluate the antimicrobial efficacy of the host-defense peptide TCP-25. Graphic abstract: Evaluation of antimicrobials using bioluminescent bacteria.

OriginalsprogEngelsk
Artikelnummere4302
TidsskriftBio-protocol
Vol/bind12
Udgave nummer2
Sider (fra-til)e4302
ISSN2331-8325
DOI
StatusUdgivet - 20 jan. 2022

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