Rapid genotypic detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis directly in clinical specimens

Didi Bang; Åse Bengård Andersen; Vibeke Østergaard Thomsen

doi:10.1128/JCM.00752-06

Rapid genotypic detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis directly in clinical specimens

Didi Bang, Åse Bengård Andersen, Vibeke Østergaard Thomsen

62 Citationer (Scopus)

Abstract

A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin (rpoB) and high-level isoniazid (katG) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.

Originalsprog	Engelsk
Tidsskrift	Journal of Clinical Microbiology
Vol/bind	44
Udgave nummer	7
Sider (fra-til)	2605-8
Antal sider	4
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.00752-06
Status	Udgivet - jul. 2006
Udgivet eksternt	Ja

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10.1128/JCM.00752-06

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title = "Rapid genotypic detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis directly in clinical specimens",

abstract = "A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin (rpoB) and high-level isoniazid (katG) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.",

keywords = "Antibiotics, Antitubercular, Antitubercular Agents, Body Fluids, Drug Resistance, Bacterial, Genotype, Humans, Isoniazid, Microbial Sensitivity Tests, Mycobacterium tuberculosis, Polymerase Chain Reaction, Rifampin, Tuberculosis, Multidrug-Resistant, Journal Article",

author = "Didi Bang and Andersen, {{\AA}se Beng{\aa}rd} and Thomsen, {Vibeke {\O}stergaard}",

year = "2006",

month = jul,

doi = "10.1128/JCM.00752-06",

language = "English",

volume = "44",

pages = "2605--8",

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TY - JOUR

T1 - Rapid genotypic detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis directly in clinical specimens

AU - Bang, Didi

AU - Andersen, Åse Bengård

AU - Thomsen, Vibeke Østergaard

PY - 2006/7

Y1 - 2006/7

N2 - A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin (rpoB) and high-level isoniazid (katG) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.

AB - A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin (rpoB) and high-level isoniazid (katG) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.

KW - Antibiotics, Antitubercular

KW - Antitubercular Agents

KW - Body Fluids

KW - Drug Resistance, Bacterial

KW - Genotype

KW - Humans

KW - Isoniazid

KW - Microbial Sensitivity Tests

KW - Mycobacterium tuberculosis

KW - Polymerase Chain Reaction

KW - Rifampin

KW - Tuberculosis, Multidrug-Resistant

KW - Journal Article

U2 - 10.1128/JCM.00752-06

DO - 10.1128/JCM.00752-06

M3 - Journal article

C2 - 16825393

SN - 0095-1137

VL - 44

SP - 2605

EP - 2608

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Rapid genotypic detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis directly in clinical specimens

Abstract

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