TY - JOUR
T1 - Rapid, automated, nonradiometric susceptibility testing of Mycobacterium tuberculosis complex to four first-line antituberculous drugs used in standard short-course chemotherapy
AU - Johansen, Isik Somuncu
AU - Thomsen, Vibeke Østergaard
AU - Marjamäki, Merja
AU - Sosnovskaja, Anaida
AU - Lundgren, Bettina
PY - 2004
Y1 - 2004
N2 - The increasing prevalence of drug-resistant tuberculosis necessitates rapid and accurate susceptibility testing. The nonradiometric BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT) system for susceptibility testing was evaluated on 222 clinical Mycobacterium tuberculosis complex isolates for isoniazid, rifampin, and ethambutol. Fifty-seven of the isolates were tested for pyrazinamide. Results were compared to those of radiometric BACTEC 460 system and discrepancies were resolved by the agar proportion method. We found an overall agreement of 99.0% for isoniazid, 99.5% for rifampin, 98.2% for ethambutol, and 100% for pyrazinamide. After resolution of discrepancies, MGIT yielded no false susceptibility for rifampin and isoniazid. Although turnaround times were comparable, MGIT provides an advantage as inoculation can be done on any weekday as the growth is monitored automatically. The automated MGIT system is a rapid and reliable alternative for susceptibility testing of M. tuberculosis complex to first-line drugs.
AB - The increasing prevalence of drug-resistant tuberculosis necessitates rapid and accurate susceptibility testing. The nonradiometric BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT) system for susceptibility testing was evaluated on 222 clinical Mycobacterium tuberculosis complex isolates for isoniazid, rifampin, and ethambutol. Fifty-seven of the isolates were tested for pyrazinamide. Results were compared to those of radiometric BACTEC 460 system and discrepancies were resolved by the agar proportion method. We found an overall agreement of 99.0% for isoniazid, 99.5% for rifampin, 98.2% for ethambutol, and 100% for pyrazinamide. After resolution of discrepancies, MGIT yielded no false susceptibility for rifampin and isoniazid. Although turnaround times were comparable, MGIT provides an advantage as inoculation can be done on any weekday as the growth is monitored automatically. The automated MGIT system is a rapid and reliable alternative for susceptibility testing of M. tuberculosis complex to first-line drugs.
KW - Antitubercular Agents
KW - Colony Count, Microbial
KW - Culture Media
KW - Drug Resistance, Multiple, Bacterial
KW - Ethambutol
KW - Humans
KW - Isoniazid
KW - Microbial Sensitivity Tests
KW - Mycobacterium tuberculosis
KW - Pyrazinamide
KW - Rifampin
KW - Sampling Studies
KW - Sensitivity and Specificity
KW - Tuberculosis, Multidrug-Resistant
U2 - 10.1016/j.diagmicrobio.2004.04.001
DO - 10.1016/j.diagmicrobio.2004.04.001
M3 - Journal article
C2 - 15474318
SN - 0732-8893
VL - 50
SP - 103
EP - 107
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 2
ER -