Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. The impact of loading, unloading, ageing and injury on the human tendon

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Blood-brain barrier permeability measured using dynamic contrast-enhanced magnetic resonance imaging: a validation study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Human brain blood flow and metabolism during isocapnic hyperoxia: the role of reactive oxygen species

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Lower body negative pressure to safely reduce intracranial pressure

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Sodium nitroprusside dilates cerebral vessels and enhances internal carotid artery flow in young men

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Molecular indicators of denervation in aging human skeletal muscle

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Muscle-strain injury exudate favors acute tissue healing and prolonged connective tissue formation in humans

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. An anti-inflammatory phenotype in visceral adipose tissue of old lean mice, augmented by exercise

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Lykke Sylow
  • Ida L Nielsen
  • Maximilian Kleinert
  • Lisbeth L V Møller
  • Thorkil Ploug
  • Peter Schjerling
  • Philip J Bilan
  • Amira Klip
  • Thomas E Jensen
  • Erik A Richter
Vis graf over relationer

KEY POINT: Exercise increases skeletal muscle energy turnover and one of the important substrates for the working muscle is glucose taken up from the blood. The GTPase Rac1 can be activated by muscle contraction and has been found to be necessary for insulin-stimulated glucose uptake, although its role in exercise-stimulated glucose uptake is unknown. We show that Rac1 regulates the translocation of the glucose transporter GLUT4 to the plasma membrane in skeletal muscle during exercise. We find that Rac1 knockout mice display significantly reduced glucose uptake in skeletal muscle during exercise.

ABSTRACT: Exercise increases skeletal muscle energy turnover and one of the important substrates for the working muscle is glucose taken up from the blood. Despite extensive efforts, the signalling mechanisms vital for glucose uptake during exercise are not yet fully understood, although the GTPase Rac1 is a candidate molecule. The present study investigated the role of Rac1 in muscle glucose uptake and substrate utilization during treadmill exercise in mice in vivo. Exercise-induced uptake of radiolabelled 2-deoxyglucose at 65% of maximum running capacity was blocked in soleus muscle and decreased by 80% and 60% in gastrocnemius and tibialis anterior muscles, respectively, in muscle-specific inducible Rac1 knockout (mKO) mice compared to wild-type littermates. By developing an assay to quantify endogenous GLUT4 translocation, we observed that GLUT4 content at the sarcolemma in response to exercise was reduced in Rac1 mKO muscle. Our findings implicate Rac1 as a regulatory element critical for controlling glucose uptake during exercise via regulation of GLUT4 translocation.

OriginalsprogEngelsk
TidsskriftThe Journal of physiology
Vol/bind594
Udgave nummer17
Sider (fra-til)4997-5008
Antal sider12
ISSN0022-3751
DOI
StatusUdgivet - 1 sep. 2016

ID: 49639680