Quantitative proteomic assessment of very early cellular signaling events

Joern Dengjel; Vyacheslav Akimov; Jesper Velgaard Olsen; Jakob Bunkenborg; Matthias Mann; Blagoy Blagoev; Jens S Andersen

doi:10.1038/nbt1301

Quantitative proteomic assessment of very early cellular signaling events

Joern Dengjel, Vyacheslav Akimov, Jesper Velgaard Olsen, Jakob Bunkenborg, Matthias Mann, Blagoy Blagoev, Jens S Andersen

99 Citationer (Scopus)

Abstract

Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

Originalsprog	Engelsk
Tidsskrift	Nature Biotechnology
Vol/bind	25
Udgave nummer	5
Sider (fra-til)	566-8
Antal sider	3
ISSN	1087-0156
DOI	https://doi.org/10.1038/nbt1301
Status	Udgivet - 2007
Udgivet eksternt	Ja

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10.1038/nbt1301

Citationsformater

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title = "Quantitative proteomic assessment of very early cellular signaling events",

abstract = "Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.",

keywords = "Fluorescence Recovery After Photobleaching, Metabolic Clearance Rate, Microscopy, Fluorescence, Phosphorylation, Proteome, Receptor, Epidermal Growth Factor, Signal Transduction, Time Factors",

author = "Joern Dengjel and Vyacheslav Akimov and Olsen, {Jesper Velgaard} and Jakob Bunkenborg and Matthias Mann and Blagoy Blagoev and Andersen, {Jens S}",

year = "2007",

doi = "10.1038/nbt1301",

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volume = "25",

pages = "566--8",

journal = "Nature Biotechnology",

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T1 - Quantitative proteomic assessment of very early cellular signaling events

AU - Dengjel, Joern

AU - Akimov, Vyacheslav

AU - Olsen, Jesper Velgaard

AU - Bunkenborg, Jakob

AU - Mann, Matthias

AU - Blagoev, Blagoy

AU - Andersen, Jens S

PY - 2007

Y1 - 2007

N2 - Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

AB - Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

KW - Fluorescence Recovery After Photobleaching

KW - Metabolic Clearance Rate

KW - Microscopy, Fluorescence

KW - Phosphorylation

KW - Proteome

KW - Receptor, Epidermal Growth Factor

KW - Signal Transduction

KW - Time Factors

U2 - 10.1038/nbt1301

DO - 10.1038/nbt1301

M3 - Journal article

C2 - 17450129

SN - 1087-0156

VL - 25

SP - 566

EP - 568

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 5

ER -

Quantitative proteomic assessment of very early cellular signaling events

Abstract

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