TY - JOUR
T1 - Quantitative immunoelectrophoretic analysis of Salmonella typhi antigens and of corresponding antibodies in human sera
AU - Espersen, F
AU - Hertz, J B
AU - Høiby, N
AU - Mogensen, H H
PY - 1980/8
Y1 - 1980/8
N2 - By quantitative immunoelectrophoretic methods, 86 different antigens were found in sonicated preparations of a Salmonella typhi, using corresponding rabbit antiserum. Lipopolysaccharide was identified, and 5 other antigens characterized. Antibodies against a total of 19 S. typhi antigens were found in human sera. The antibody response in patients with typhoid fever was significantly more pronounced compared to that in normal persons. When the antibody response was expressed as a precipitin score, the predictive value of both a positive (precipitin score greater than or equal to 8) test and a negative test was 100%, when sera from patients suffering from typhoid fever were compared with sera from normal persons.
AB - By quantitative immunoelectrophoretic methods, 86 different antigens were found in sonicated preparations of a Salmonella typhi, using corresponding rabbit antiserum. Lipopolysaccharide was identified, and 5 other antigens characterized. Antibodies against a total of 19 S. typhi antigens were found in human sera. The antibody response in patients with typhoid fever was significantly more pronounced compared to that in normal persons. When the antibody response was expressed as a precipitin score, the predictive value of both a positive (precipitin score greater than or equal to 8) test and a negative test was 100%, when sera from patients suffering from typhoid fever were compared with sera from normal persons.
KW - Animals
KW - Antibodies, Bacterial/analysis
KW - Antigens, Bacterial/analysis
KW - Epitopes
KW - Humans
KW - Immunoelectrophoresis, Two-Dimensional
KW - Lipopolysaccharides/analysis
KW - Precipitins/analysis
KW - Rabbits
KW - Salmonella typhi/immunology
KW - Typhoid Fever/immunology
U2 - 10.1111/j.1699-0463.1980.tb02634.x
DO - 10.1111/j.1699-0463.1980.tb02634.x
M3 - Journal article
C2 - 6158255
SN - 0105-0656
VL - 88
SP - 237
EP - 242
JO - Acta pathologica et microbiologica Scandinavica. Section B, Microbiology
JF - Acta pathologica et microbiologica Scandinavica. Section B, Microbiology
IS - 4
ER -