Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen; Lisbeth Nielsen Fink; Carsten Struve; Claus Sternberg; Jens Bo Andersen; Jørn Brynskov; Ole Haagen Nielsen; Susanne Brix

doi:10.1016/j.mimet.2011.04.002

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Stina Rikke Jensen
, Lisbeth Nielsen Fink
, Carsten Struve
, Claus Sternberg
, Jens Bo Andersen
, Jørn Brynskov
, Ole Haagen Nielsen
, Susanne Brix

8 Citationer (Scopus)

Abstract

We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

Originalsprog	Engelsk
Tidsskrift	Journal of Microbiological Methods
Vol/bind	86
Udgave nummer	1
Sider (fra-til)	111-4
Antal sider	4
ISSN	0167-7012
DOI	https://doi.org/10.1016/j.mimet.2011.04.002
Status	Udgivet - 2011

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10.1016/j.mimet.2011.04.002

Citationsformater

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title = "Quantification of specific E. coli in gut mucosa from Crohn's disease patients",

abstract = "We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.",

keywords = "Crohn Disease, DNA Primers, Escherichia coli, Humans, Intestinal Mucosa, Polymerase Chain Reaction",

author = "Jensen, \{Stina Rikke\} and Fink, \{Lisbeth Nielsen\} and Carsten Struve and Claus Sternberg and Andersen, \{Jens Bo\} and J{\o}rn Brynskov and Nielsen, \{Ole Haagen\} and Susanne Brix",

year = "2011",

doi = "10.1016/j.mimet.2011.04.002",

language = "English",

volume = "86",

pages = "111--4",

journal = "Journal of Microbiological Methods",

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TY - JOUR

T1 - Quantification of specific E. coli in gut mucosa from Crohn's disease patients

AU - Jensen, Stina Rikke

AU - Fink, Lisbeth Nielsen

AU - Struve, Carsten

AU - Sternberg, Claus

AU - Andersen, Jens Bo

AU - Brynskov, Jørn

AU - Nielsen, Ole Haagen

AU - Brix, Susanne

PY - 2011

Y1 - 2011

N2 - We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

AB - We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

KW - Crohn Disease

KW - DNA Primers

KW - Escherichia coli

KW - Humans

KW - Intestinal Mucosa

KW - Polymerase Chain Reaction

U2 - 10.1016/j.mimet.2011.04.002

DO - 10.1016/j.mimet.2011.04.002

M3 - Journal article

C2 - 21504765

SN - 0167-7012

VL - 86

SP - 111

EP - 114

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

Abstract

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