Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Quantification of biotin in plasma samples by column switching liquid chromatography - tandem mass spectrometry

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Prognostic utility of serum YKL-40 in patients with cervical cancer

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Estimation of the celiac disease prevalence in Denmark and the diagnostic value of HLA-DQ2/DQ8

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Circadian variations in plasma concentrations of cholecystokinin and gastrin in man

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Chromogranin A in cardiovascular endocrinology

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Pharmacological but not physiological GDF15 suppresses feeding and the motivation to exercise

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Gastric Residual to Predict Necrotizing Enterocolitis in Preterm Piglets As Models for Infants

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Biotin (or Vitamin B7) is a vitamin where deficiency can be caused by inadequate intake. Biotin deficiency is rare, as most people get enough biotin from diet, since many foods contain biotin. In addition to biotin from food, intestinal bacteria can synthesize biotin, which can then be absorbed by the body. Supplementation with biotin has been advocated, mainly due to proposed beneficial effects on skin, nail and hair growth. There is no evidence that high biotin intakes are toxic, but a high intake may interfere with diagnostic assays that use biotin-streptavidin technology. These tests are commonly used to measure plasma concentrations of a wide range of hormones. Erroneous results may lead to misdiagnosis of various endocrine disorders. Supplementation with high-dose biotin has been used experimental for the treatment of diseases (e.g. multiple sclerosis) and high doses are used to obtain effect on nail and hair growth. On this background a demand for tests to determine if there is a risk of obtaining false test results when using biotin-streptavidin based tests have appeared. In this paper we present a method based on column switching liquid chromatography tandem mass spectrometry for the quantification of biotin in plasma and serum and explore the effects of biotin on an immunoassay based on biotin strept(avidin) chemistry.

OriginalsprogEngelsk
TidsskriftScandinavian Journal of Clinical and Laboratory Investigation
Vol/bind81
Udgave nummer2
Sider (fra-til)127-136
Antal sider10
ISSN0036-5513
DOI
StatusUdgivet - apr. 2021

ID: 62270487