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Quantification of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-7,8-dihydro-guanosine concentrations in urine and plasma for estimating 24-h urinary output

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@article{1c150119de9b402ea67cb41124514875,
title = "Quantification of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-7,8-dihydro-guanosine concentrations in urine and plasma for estimating 24-h urinary output",
abstract = "Among markers for oxidative stress urinary excretion 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo) have been widely used in controlled and epidemiological studies, and are considered to represent intracellular markers of oxidation of DNA and RNA in the entire organism, respectively. Although being non-invasive, urinary methods have shortcomings. There is no established method for analysis of 8-oxodGuo and 8-oxoGuo in plasma and the few plasma values presented in the literature vary greatly. We here present a liquid chromatography mass spectrometry method with full validation for analysis of 8-oxodGuo and 8-oxoGuo in plasma. Further, we investigated the basis for our previously physiological model and show that a single plasma sample can be used to estimate the 24-h production of 8-oxoGuo, whereas we challenge the use of urinary 8-oxodGuo/creatinine ratio or plasma 8-oxodGuo as measures of oxidative stress.",
keywords = "DNA oxidation, Mass spectroscopy, Oxidative stress, Plasma, RNA oxidation, Urine",
author = "Trine Henriksen and Allan Weimann and Larsen, {Emil List} and Poulsen, {Henrik Enghusen}",
note = "Copyright {\textcopyright} 2021 Elsevier Inc. All rights reserved.",
year = "2021",
month = aug,
day = "20",
doi = "10.1016/j.freeradbiomed.2021.06.014",
language = "English",
volume = "172",
pages = "350--357",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Science Publishing Co., Inc. (New York)",

}

RIS

TY - JOUR

T1 - Quantification of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-7,8-dihydro-guanosine concentrations in urine and plasma for estimating 24-h urinary output

AU - Henriksen, Trine

AU - Weimann, Allan

AU - Larsen, Emil List

AU - Poulsen, Henrik Enghusen

N1 - Copyright © 2021 Elsevier Inc. All rights reserved.

PY - 2021/8/20

Y1 - 2021/8/20

N2 - Among markers for oxidative stress urinary excretion 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo) have been widely used in controlled and epidemiological studies, and are considered to represent intracellular markers of oxidation of DNA and RNA in the entire organism, respectively. Although being non-invasive, urinary methods have shortcomings. There is no established method for analysis of 8-oxodGuo and 8-oxoGuo in plasma and the few plasma values presented in the literature vary greatly. We here present a liquid chromatography mass spectrometry method with full validation for analysis of 8-oxodGuo and 8-oxoGuo in plasma. Further, we investigated the basis for our previously physiological model and show that a single plasma sample can be used to estimate the 24-h production of 8-oxoGuo, whereas we challenge the use of urinary 8-oxodGuo/creatinine ratio or plasma 8-oxodGuo as measures of oxidative stress.

AB - Among markers for oxidative stress urinary excretion 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo) have been widely used in controlled and epidemiological studies, and are considered to represent intracellular markers of oxidation of DNA and RNA in the entire organism, respectively. Although being non-invasive, urinary methods have shortcomings. There is no established method for analysis of 8-oxodGuo and 8-oxoGuo in plasma and the few plasma values presented in the literature vary greatly. We here present a liquid chromatography mass spectrometry method with full validation for analysis of 8-oxodGuo and 8-oxoGuo in plasma. Further, we investigated the basis for our previously physiological model and show that a single plasma sample can be used to estimate the 24-h production of 8-oxoGuo, whereas we challenge the use of urinary 8-oxodGuo/creatinine ratio or plasma 8-oxodGuo as measures of oxidative stress.

KW - DNA oxidation

KW - Mass spectroscopy

KW - Oxidative stress

KW - Plasma

KW - RNA oxidation

KW - Urine

UR - http://www.scopus.com/inward/record.url?scp=85108819798&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2021.06.014

DO - 10.1016/j.freeradbiomed.2021.06.014

M3 - Journal article

C2 - 34166769

VL - 172

SP - 350

EP - 357

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

ER -

ID: 66834355