Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Purification of porcine aminoterminal propeptide of type III procollagen from lymph and use for lymphatic clearance studies in pigs.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

  1. Kontroll er bra-men tillit er bedre

    Publikation: Bidrag til tidsskriftTidsskriftartikelFormidling

  2. Mentalisering på arbejde i organisationer

    Publikation: Bidrag til tidsskriftTidsskriftartikelFormidling

  3. Omsorg for nybegynder supervisanden i kognitiv terapi supervision

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. (U)overenstemmelse mellem behandler- og patient-vurdering af effekt af gruppeterapi.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Effects of transjugular intrahepatic portosystemic shunt (TIPS) on blood volume distribution in patients with cirrhosis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The Royal Free Hospital Cirrhosis Glomerular Filtration Rate: Validation in a Danish Cohort

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Red blood cell sodium transport in patients with cirrhosis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer
To investigate the lymphatic transport of the aminoterminal propeptide of type III procollagen (PIIINP) we established a thoracic duct-venous shunt in 6 pigs. Porcine PIIINP was purified, characterised, and compared with human PIIINP to ensure the suitability of the radioimmunoassay of human PIIINP for measurements in pigs. SDS-PAGE and radioimmunoinhibition assays show human and porcine PIIINP to be similar, thus indicating that the assay of human PIIINP is also reliable for determinations on pig serum and lymph. Intact PIIINP, as identified by gel filtration, accounted for 60% and 40% of the total PIIINP immunoreactivity in lymph and serum, respectively. The higher amount of total immunoreactivity and proportion of intact PIIINP in lymph compared with serum support the hypothesis that intact PIIINP is transported from peripheral tissue into the circulation by lymph. Two days after the shunt was established, the lymph was collected quantitatively hour-by-hour for 24 h. The flow was higher during the light periods than in the dark (p less than 0.01). The PIIINP concentration varied inversely with the flow, being higher in the dark hours (p less than 0.03). However, the total collected amount of PIIINP in lymph did not differ during the light and dark periods. Serum PIIINP remained unchanged over the 24 h. The lymphatic clearance of total PIIINP immunoreactive components was 6.2 ml serum/min and the lymphatic clearance of intact PIIINP was 9.1 ml serum/min, equal to 7 and 10 times the plasma volume/24 h, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Bidragets oversatte titelPurification of porcine aminoterminal propeptide of type III procollagen from lymph and use for lymphatic clearance studies in pigs.
OriginalsprogEngelsk
TidsskriftMatrix
Vol/bind12
Udgave nummer1
Sider (fra-til)73-79
Antal sider7
ISSN0934-8832
StatusUdgivet - 1992

ID: 32556331