@article{8f63c69d8cc24ab9acdb99203d15539b,
title = "Purification of human serum hyaluronidase using chromatofocusing",
abstract = "A commercial chromatofocusing system was applied to Cohn's fraction III of human serum to purify hyaluronidase (E.C.3.2.1.3.5). The protein that eluted in the pH range 4.7-5.3 was pooled and precipitated by adding ammonium sulphate to 50% saturation. This sequence of fractionation purified hyaluronidase extensively by immunological criteria. It is shown that hyaluronidase is a population of enzymes displaying microheterogeneity. The commercial chromatofocusing system behaved as theoretically expected. The capacity of the gel is 3 mg per ml gel. Any overload will be trapped or precipitated in the gel. The gel is easy to handle and did not deteriorate on repeated use.",
keywords = "Humans, Hyaluronoglucosaminidase, Immunoelectrophoresis, Isoelectric Focusing, Reference Values",
author = "M Fenger",
year = "1982",
language = "English",
volume = "240",
pages = "173--9",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier BV",
number = "1",
}