Pseudomonas aeruginosa biofilm aggravates skin inflammatory response in BALB/c mice in a novel chronic wound model

Hannah Trøstrup, Kim Thomsen, Lars J Christophersen, Hans Petter Hougen, Thomas Bjarnsholt, Peter Østrup Jensen, Nikolai Kirkby, Henrik Calum, Niels Høiby, Claus Moser

61 Citationer (Scopus)

Abstract

Chronic wounds are presumed to persist in the inflammatory state, preventing healing. Emerging evidence indicates a clinical impact of bacterial biofilms in soft tissues, including Pseudomonas aeruginosa (PA) biofilms. To further investigate this, we developed a chronic PA biofilm wound infection model in C3H/HeN and BALB/c mice. The chronic wound was established by an injection of seaweed alginate-embedded P. aeruginosa PAO1 beneath a third-degree thermal lesion providing full thickness skin necrosis, as in human chronic wounds. Cultures revealed growth of PA, and both alginate with or without PAO1 generated a polymorphonuclear-dominated inflammation early after infection. However, both at days 4 and 7, there were a more acute polymorphonuclear-dominated and higher degree of inflammation in the PAO1 containing group (p
OriginalsprogEngelsk
TidsskriftWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Vol/bind21
Udgave nummer2
Sider (fra-til)292-9
Antal sider8
ISSN1067-1927
DOI
StatusUdgivet - 2013

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