Abstract
Chronic wounds are presumed to persist in the inflammatory state, preventing healing. Emerging evidence indicates a clinical impact of bacterial biofilms in soft tissues, including Pseudomonas aeruginosa (PA) biofilms. To further investigate this, we developed a chronic PA biofilm wound infection model in C3H/HeN and BALB/c mice. The chronic wound was established by an injection of seaweed alginate-embedded P. aeruginosa PAO1 beneath a third-degree thermal lesion providing full thickness skin necrosis, as in human chronic wounds. Cultures revealed growth of PA, and both alginate with or without PAO1 generated a polymorphonuclear-dominated inflammation early after infection. However, both at days 4 and 7, there were a more acute polymorphonuclear-dominated and higher degree of inflammation in the PAO1 containing group (p
Originalsprog | Engelsk |
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Tidsskrift | Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society |
Vol/bind | 21 |
Udgave nummer | 2 |
Sider (fra-til) | 292-9 |
Antal sider | 8 |
ISSN | 1067-1927 |
DOI | |
Status | Udgivet - 2013 |