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Progressive changes in human follicular fluid composition over the course of ovulation: quantitative proteomic analyses

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Poulsen, Liv la Cour ; Pla, Indira ; Sanchez, Aniel ; Grøndahl, Marie Louise ; Marko-Varga, György ; Yding Andersen, Claus ; Englund, Anne Lis Mikkelsen ; Malm, Johan. / Progressive changes in human follicular fluid composition over the course of ovulation : quantitative proteomic analyses. I: Molecular and Cellular Endocrinology. 2019 ; Bind 495. s. 110522.

Bibtex

@article{ed8ae5928ca94e7ea919454d1ad4a36d,
title = "Progressive changes in human follicular fluid composition over the course of ovulation: quantitative proteomic analyses",
abstract = "Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. In total, 400 proteins were identified and the levels of 40 proteins changed significantly across ovulation, evaluated by analysis of covariance (adjusted p < 0.05) and on-off expression patterns. The majority peaked after 12-17 h, e.g., AREG (p < 0.0001), TNFAIP6 (p < 0.0001), and LDHB (p = 0.0316), while some increased to peak after 36 h e.g., ACPP (p < 0.0001), TIMP1 (p < 0.0001) and SERPINE1 (p = 0.0002). Collectively, this study highlights proteins and pathways of importance for ovulation and oocyte competence in humans.",
author = "Poulsen, {Liv la Cour} and Indira Pla and Aniel Sanchez and Gr{\o}ndahl, {Marie Louise} and Gy{\"o}rgy Marko-Varga and {Yding Andersen}, Claus and Englund, {Anne Lis Mikkelsen} and Johan Malm",
note = "Copyright {\textcopyright} 2019 Elsevier B.V. All rights reserved.",
year = "2019",
month = sep,
day = "15",
doi = "10.1016/j.mce.2019.110522",
language = "English",
volume = "495",
pages = "110522",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - Progressive changes in human follicular fluid composition over the course of ovulation

T2 - quantitative proteomic analyses

AU - Poulsen, Liv la Cour

AU - Pla, Indira

AU - Sanchez, Aniel

AU - Grøndahl, Marie Louise

AU - Marko-Varga, György

AU - Yding Andersen, Claus

AU - Englund, Anne Lis Mikkelsen

AU - Malm, Johan

N1 - Copyright © 2019 Elsevier B.V. All rights reserved.

PY - 2019/9/15

Y1 - 2019/9/15

N2 - Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. In total, 400 proteins were identified and the levels of 40 proteins changed significantly across ovulation, evaluated by analysis of covariance (adjusted p < 0.05) and on-off expression patterns. The majority peaked after 12-17 h, e.g., AREG (p < 0.0001), TNFAIP6 (p < 0.0001), and LDHB (p = 0.0316), while some increased to peak after 36 h e.g., ACPP (p < 0.0001), TIMP1 (p < 0.0001) and SERPINE1 (p = 0.0002). Collectively, this study highlights proteins and pathways of importance for ovulation and oocyte competence in humans.

AB - Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. In total, 400 proteins were identified and the levels of 40 proteins changed significantly across ovulation, evaluated by analysis of covariance (adjusted p < 0.05) and on-off expression patterns. The majority peaked after 12-17 h, e.g., AREG (p < 0.0001), TNFAIP6 (p < 0.0001), and LDHB (p = 0.0316), while some increased to peak after 36 h e.g., ACPP (p < 0.0001), TIMP1 (p < 0.0001) and SERPINE1 (p = 0.0002). Collectively, this study highlights proteins and pathways of importance for ovulation and oocyte competence in humans.

U2 - 10.1016/j.mce.2019.110522

DO - 10.1016/j.mce.2019.110522

M3 - Journal article

C2 - 31356852

VL - 495

SP - 110522

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

ER -

ID: 59045181