Abstract
OBJECTIVE: To assess the production of fibrogenic cytokines by interleukin-2 (IL-2)-stimulated peripheral blood leukocytes and to examine their ability to stimulate the production of connective tissue.
METHODS: Culture medium from human peripheral blood leukocytes incubated with or without IL-2 was tested for induction of fibroblast proliferation, collagen synthesis, and expression of cytokine genes.
RESULTS: Supernatants from IL-2-treated peripheral blood leukocytes induced six times more fibroblast proliferation than medium from leukocytes cultured without IL-2. The expression of type I procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment.
CONCLUSION: Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Obstetrics and Gynecology |
| Vol/bind | 82 |
| Udgave nummer | 1 |
| Sider (fra-til) | 29-36 |
| Antal sider | 8 |
| ISSN | 0029-7844 |
| Status | Udgivet - jul. 1993 |
| Udgivet eksternt | Ja |