Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Processing-independent analysis (PIA): a method for quantitation of the total peptide-gene expression

Publikation: Bidrag til tidsskriftReviewpeer review

  1. Post-oral fat-induced satiation is mediated by endogenous CCK and GLP-1 in a fat self-administration mouse model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Association between habitual sleep duration/quality and appetite markers in individuals with obesity

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Plasma volume contraction reduces atrial natriuretic peptide after four days of hypobaric hypoxia exposure

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Chromogranin A in cardiovascular endocrinology

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

The translational product of protein-coding genes undergoes extensive posttranslational modifications. The modifications ensure an increased molecular and functional diversity at protein- and peptide-level. Prohormones are small pro-proteins that are expressed in many cell types, for instance endocrine cells, immune cells, myocytes and neurons. Here they mature to bioactive peptides (cytokines, hormones, growth factors, and neurotransmitters) that are released from the cells in an often regulated manner. The posttranslational processing of prohormones is cell-specific, however, and may vary during evolution and disease. Therefore, it is often inadequate to measure just a single peptide fragment as marker of endocrine, immune, and neuronal functions. In order to meet this challenge, we developed years back a simple "processing-independent analysis" (PIA) for accurate quantification of the total pro-protein product - irrespective of the degree and nature of the posttranslational processing. This review provides an overview of the PIA principle and describes examples of PIA results in different peptide systems.

OriginalsprogEngelsk
Artikelnummer170427
TidsskriftPeptides
Vol/bind135
ISSN0196-9781
DOI
StatusUdgivet - jan. 2021

Bibliografisk note

Copyright © 2020 Elsevier Inc. All rights reserved.

ID: 62406327