TY - JOUR
T1 - Prevalence of hiv infection in seronegative high-risk individuals examined by virus isolation and pcr
AU - Nielsen, Claus
AU - Teglbjaerg, Lars Stubbe
AU - Pedersen, Court
AU - Lundgren, Jens Dilling
AU - Nielsen, Carsten Møller
AU - Vestergaard, Bent Faber
PY - 1991/11
Y1 - 1991/11
N2 - HIV seronegative individuals with high-risk behavior were tested for HIV infection by sensitive virus isolation techniques using T4 lymphocytes and monocyte/macrophages, and by detection of proviral DNA using PCR with three different sets of nested primers. No evidence of HIV infection was found among the 31 seronegative high-risk subjects, either by virus isolation of by PCR (97.5% confidence limits, 0-11). Our results indicate that ongoing HIV infection in seronegative persons at high risk of infection is a rare event.
AB - HIV seronegative individuals with high-risk behavior were tested for HIV infection by sensitive virus isolation techniques using T4 lymphocytes and monocyte/macrophages, and by detection of proviral DNA using PCR with three different sets of nested primers. No evidence of HIV infection was found among the 31 seronegative high-risk subjects, either by virus isolation of by PCR (97.5% confidence limits, 0-11). Our results indicate that ongoing HIV infection in seronegative persons at high risk of infection is a rare event.
KW - High-risk sexual activity
KW - HIV-1
KW - Isolation
KW - Macrophages
KW - Polymerase chain reaction
KW - Seronegative
KW - T4-cells
UR - http://www.scopus.com/inward/record.url?scp=0025720627&partnerID=8YFLogxK
M3 - Journal article
C2 - 1684386
AN - SCOPUS:0025720627
SN - 1525-4135
VL - 4
SP - 1107
EP - 1111
JO - Journal of Acquired Immune Deficiency Syndromes
JF - Journal of Acquired Immune Deficiency Syndromes
IS - 11
ER -