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Predicting respiratory distress syndrome at birth using fast test based on spectroscopy of gastric aspirates. 1. Biochemical part

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Schousboe, P, Verder, H, Jessen, TE, Heiring, C, Bender, L, Ebbesen, F, Dahl, M, Eschen, C, Fenger-Grøn, J, Höskuldsson, A, Reinholdt, J, Scoutaris, N & Smedegaard, H 2020, 'Predicting respiratory distress syndrome at birth using fast test based on spectroscopy of gastric aspirates. 1. Biochemical part' Acta paediatrica, bind 109, nr. 2, s. 280-284. https://doi.org/10.1111/apa.14896

APA

CBE

Schousboe P, Verder H, Jessen TE, Heiring C, Bender L, Ebbesen F, Dahl M, Eschen C, Fenger-Grøn J, Höskuldsson A, Reinholdt J, Scoutaris N, Smedegaard H. 2020. Predicting respiratory distress syndrome at birth using fast test based on spectroscopy of gastric aspirates. 1. Biochemical part. Acta paediatrica. 109(2):280-284. https://doi.org/10.1111/apa.14896

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Author

Schousboe, Peter ; Verder, Henrik ; Jessen, Torben E ; Heiring, Christian ; Bender, Lars ; Ebbesen, Finn ; Dahl, Marianne ; Eschen, Christian ; Fenger-Grøn, Jesper ; Höskuldsson, Agnar ; Reinholdt, Jes ; Scoutaris, Nikolaos ; Smedegaard, Heidi. / Predicting respiratory distress syndrome at birth using fast test based on spectroscopy of gastric aspirates. 1. Biochemical part. I: Acta paediatrica. 2020 ; Bind 109, Nr. 2. s. 280-284.

Bibtex

@article{394e164df2a84a4493c664cdc4da4833,
title = "Predicting respiratory distress syndrome at birth using fast test based on spectroscopy of gastric aspirates. 1. Biochemical part",
abstract = "Aim: To develop a fast bedside lung maturity test. Methods: Gastric aspirates obtained from premature infants contain lamellar bodies, carrying lung surfactant. To estimate lung maturity, we isolated lamellar bodies from fresh gastric aspirates by centrifugation. Erythrocytes and other cells were lysed by adding water and discarded subsequently with the supernatant. Mid-infrared spectroscopy was then performed to measure the lung maturity as lecithin–sphingomyelin ratio. Lecithin was determined as dipalmitoylphosphatidylcholine, the most surface-active phospholipid. Algorithms to measure lecithin and sphingomyelin concentrations in fresh gastric aspirates were developed on aspirates from 140 premature infants. Each gastric aspirate sample was divided into two samples: one for mass spectrometry as reference and one for spectroscopy. Development of the algorithm is described in detail in Appendix S1. Results: Gastric aspirates stored at 4–5°C avoid flocculation of proteins and phospholipids in contrast to when the aspirates were frozen and thawed. Omission of freezing and concentration of the lung surfactant by centrifugation combined with diminished influence of proteins improves the spectroscopic measurement of lecithin–sphingomyelin ratio. Measurement of lecithin–sphingomyelin ratio by the new method was performed within 10–15 minutes. Conclusion: We present a new fast bedside lung maturity test on fresh gastric aspirate for early targeted surfactant treatment.",
keywords = "Gastric aspirate, Lamellar bodies, Lecithin–sphingomyelin ratio (L/S), Mid-infrared spectroscopy, Prematurity, Respiratory distress syndrome",
author = "Peter Schousboe and Henrik Verder and Jessen, {Torben E} and Christian Heiring and Lars Bender and Finn Ebbesen and Marianne Dahl and Christian Eschen and Jesper Fenger-Gr{\o}n and Agnar H{\"o}skuldsson and Jes Reinholdt and Nikolaos Scoutaris and Heidi Smedegaard",
note = "{\circledC}2019 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.",
year = "2020",
month = "2",
doi = "10.1111/apa.14896",
language = "English",
volume = "109",
pages = "280--284",
journal = "Acta paediatrica",
issn = "1651-2227",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Predicting respiratory distress syndrome at birth using fast test based on spectroscopy of gastric aspirates. 1. Biochemical part

AU - Schousboe, Peter

AU - Verder, Henrik

AU - Jessen, Torben E

AU - Heiring, Christian

AU - Bender, Lars

AU - Ebbesen, Finn

AU - Dahl, Marianne

AU - Eschen, Christian

AU - Fenger-Grøn, Jesper

AU - Höskuldsson, Agnar

AU - Reinholdt, Jes

AU - Scoutaris, Nikolaos

AU - Smedegaard, Heidi

N1 - ©2019 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.

PY - 2020/2

Y1 - 2020/2

N2 - Aim: To develop a fast bedside lung maturity test. Methods: Gastric aspirates obtained from premature infants contain lamellar bodies, carrying lung surfactant. To estimate lung maturity, we isolated lamellar bodies from fresh gastric aspirates by centrifugation. Erythrocytes and other cells were lysed by adding water and discarded subsequently with the supernatant. Mid-infrared spectroscopy was then performed to measure the lung maturity as lecithin–sphingomyelin ratio. Lecithin was determined as dipalmitoylphosphatidylcholine, the most surface-active phospholipid. Algorithms to measure lecithin and sphingomyelin concentrations in fresh gastric aspirates were developed on aspirates from 140 premature infants. Each gastric aspirate sample was divided into two samples: one for mass spectrometry as reference and one for spectroscopy. Development of the algorithm is described in detail in Appendix S1. Results: Gastric aspirates stored at 4–5°C avoid flocculation of proteins and phospholipids in contrast to when the aspirates were frozen and thawed. Omission of freezing and concentration of the lung surfactant by centrifugation combined with diminished influence of proteins improves the spectroscopic measurement of lecithin–sphingomyelin ratio. Measurement of lecithin–sphingomyelin ratio by the new method was performed within 10–15 minutes. Conclusion: We present a new fast bedside lung maturity test on fresh gastric aspirate for early targeted surfactant treatment.

AB - Aim: To develop a fast bedside lung maturity test. Methods: Gastric aspirates obtained from premature infants contain lamellar bodies, carrying lung surfactant. To estimate lung maturity, we isolated lamellar bodies from fresh gastric aspirates by centrifugation. Erythrocytes and other cells were lysed by adding water and discarded subsequently with the supernatant. Mid-infrared spectroscopy was then performed to measure the lung maturity as lecithin–sphingomyelin ratio. Lecithin was determined as dipalmitoylphosphatidylcholine, the most surface-active phospholipid. Algorithms to measure lecithin and sphingomyelin concentrations in fresh gastric aspirates were developed on aspirates from 140 premature infants. Each gastric aspirate sample was divided into two samples: one for mass spectrometry as reference and one for spectroscopy. Development of the algorithm is described in detail in Appendix S1. Results: Gastric aspirates stored at 4–5°C avoid flocculation of proteins and phospholipids in contrast to when the aspirates were frozen and thawed. Omission of freezing and concentration of the lung surfactant by centrifugation combined with diminished influence of proteins improves the spectroscopic measurement of lecithin–sphingomyelin ratio. Measurement of lecithin–sphingomyelin ratio by the new method was performed within 10–15 minutes. Conclusion: We present a new fast bedside lung maturity test on fresh gastric aspirate for early targeted surfactant treatment.

KW - Gastric aspirate

KW - Lamellar bodies

KW - Lecithin–sphingomyelin ratio (L/S)

KW - Mid-infrared spectroscopy

KW - Prematurity

KW - Respiratory distress syndrome

U2 - 10.1111/apa.14896

DO - 10.1111/apa.14896

M3 - Journal article

VL - 109

SP - 280

EP - 284

JO - Acta paediatrica

JF - Acta paediatrica

SN - 1651-2227

IS - 2

ER -

ID: 57375948