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Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

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@article{fe0e71f71d5e41aa9d7d3cb45e696de0,
title = "Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene",
abstract = "We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcus aureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.",
keywords = "blaZ, In vitro susceptibility testing, PSSA PRSA, Staphylococcus aureus",
author = "Eriksen, {Helle Brander} and Andreas Petersen and Michael Pedersen and S{\o}ren Overballe-Petersen and Larsen, {Anders Rhod} and Holzknecht, {Barbara Juliane}",
note = "{\textcopyright} 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",
year = "2021",
month = sep,
day = "16",
doi = "10.1007/s10096-021-04344-w",
language = "English",
journal = "European Journal of Clinical Microbiology and Infectious Diseases",
issn = "0934-9723",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

AU - Eriksen, Helle Brander

AU - Petersen, Andreas

AU - Pedersen, Michael

AU - Overballe-Petersen, Søren

AU - Larsen, Anders Rhod

AU - Holzknecht, Barbara Juliane

N1 - © 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2021/9/16

Y1 - 2021/9/16

N2 - We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcus aureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.

AB - We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcus aureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.

KW - blaZ

KW - In vitro susceptibility testing

KW - PSSA PRSA

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=85115057061&partnerID=8YFLogxK

U2 - 10.1007/s10096-021-04344-w

DO - 10.1007/s10096-021-04344-w

M3 - Journal article

C2 - 34529167

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

SN - 0934-9723

ER -

ID: 67655364