Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis

Julie Hejl; Klaus Theede; Brian Møllgren; Kirsten Vikkelsø Madsen; Ashraf Heidari; Anna Á Steig; Mogens Fenger

doi:10.1016/j.plabm.2017.11.002

Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis

Julie Hejl, Klaus Theede, Brian Møllgren, Kirsten Vikkelsø Madsen, Ashraf Heidari, Anna Á Steig, Mogens Fenger

19 Citationer (Scopus)

Abstract

Objectives: Fecal calprotectin (FC) is widely used to monitor the activity of inflammatory bowel disease (IBD) and to tailor medical treatment to disease activity. Laboratory testing of fecal samples may have a turnaround time of 1-2 weeks, whereas FC home testing allows results within hours and thus enables a rapid response to clinical deterioration.

Design and methods: Fifty-five stool samples were analyzed by the IBDoc® Calprotectin Home Testing kit and the BÜHLMANN fCAL® turbo assay on a Roche Cobas 6000 c501. The correlation between the assays was assessed using Spearman's Rho correlation coefficient and the intermediate imprecision of both assays was calculated.

Results: We found a strong correlation coefficient of 0.887 between FC measured on IBDoc® and the laboratory assay BÜHLMANN fCAL® turbo. The coefficients of variation (CVs) at three different FC levels were in the range 2.3-5.5% (BÜHLMANN fCAL® turbo) and in the range of 4.8-26.6% (IBDoc®).

Conclusions: This study suggests that IBDoc® is a suitable alternative for the assessment of disease activity in IBD patients.

Originalsprog	Engelsk
Tidsskrift	Practical Laboratory Medicine
Vol/bind	10
Sider (fra-til)	10-14
ISSN	2352-5517
DOI	https://doi.org/10.1016/j.plabm.2017.11.002
Status	Udgivet - mar. 2018

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10.1016/j.plabm.2017.11.002

Citationsformater

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title = "Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis",

abstract = "Objectives: Fecal calprotectin (FC) is widely used to monitor the activity of inflammatory bowel disease (IBD) and to tailor medical treatment to disease activity. Laboratory testing of fecal samples may have a turnaround time of 1-2 weeks, whereas FC home testing allows results within hours and thus enables a rapid response to clinical deterioration.Design and methods: Fifty-five stool samples were analyzed by the IBDoc{\textregistered} Calprotectin Home Testing kit and the B{\"U}HLMANN fCAL{\textregistered} turbo assay on a Roche Cobas 6000 c501. The correlation between the assays was assessed using Spearman's Rho correlation coefficient and the intermediate imprecision of both assays was calculated.Results: We found a strong correlation coefficient of 0.887 between FC measured on IBDoc{\textregistered} and the laboratory assay B{\"U}HLMANN fCAL{\textregistered} turbo. The coefficients of variation (CVs) at three different FC levels were in the range 2.3-5.5% (B{\"U}HLMANN fCAL{\textregistered} turbo) and in the range of 4.8-26.6% (IBDoc{\textregistered}).Conclusions: This study suggests that IBDoc{\textregistered} is a suitable alternative for the assessment of disease activity in IBD patients.",

keywords = "Journal Article",

author = "Julie Hejl and Klaus Theede and Brian M{\o}llgren and Madsen, {Kirsten Vikkels{\o}} and Ashraf Heidari and {{\'A} Steig}, Anna and Mogens Fenger",

year = "2018",

month = mar,

doi = "10.1016/j.plabm.2017.11.002",

language = "English",

volume = "10",

pages = "10--14",

journal = "Practical Laboratory Medicine",

issn = "2352-5517",

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TY - JOUR

T1 - Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis

AU - Hejl, Julie

AU - Theede, Klaus

AU - Møllgren, Brian

AU - Madsen, Kirsten Vikkelsø

AU - Heidari, Ashraf

AU - Á Steig, Anna

AU - Fenger, Mogens

PY - 2018/3

Y1 - 2018/3

N2 - Objectives: Fecal calprotectin (FC) is widely used to monitor the activity of inflammatory bowel disease (IBD) and to tailor medical treatment to disease activity. Laboratory testing of fecal samples may have a turnaround time of 1-2 weeks, whereas FC home testing allows results within hours and thus enables a rapid response to clinical deterioration.Design and methods: Fifty-five stool samples were analyzed by the IBDoc® Calprotectin Home Testing kit and the BÜHLMANN fCAL® turbo assay on a Roche Cobas 6000 c501. The correlation between the assays was assessed using Spearman's Rho correlation coefficient and the intermediate imprecision of both assays was calculated.Results: We found a strong correlation coefficient of 0.887 between FC measured on IBDoc® and the laboratory assay BÜHLMANN fCAL® turbo. The coefficients of variation (CVs) at three different FC levels were in the range 2.3-5.5% (BÜHLMANN fCAL® turbo) and in the range of 4.8-26.6% (IBDoc®).Conclusions: This study suggests that IBDoc® is a suitable alternative for the assessment of disease activity in IBD patients.

AB - Objectives: Fecal calprotectin (FC) is widely used to monitor the activity of inflammatory bowel disease (IBD) and to tailor medical treatment to disease activity. Laboratory testing of fecal samples may have a turnaround time of 1-2 weeks, whereas FC home testing allows results within hours and thus enables a rapid response to clinical deterioration.Design and methods: Fifty-five stool samples were analyzed by the IBDoc® Calprotectin Home Testing kit and the BÜHLMANN fCAL® turbo assay on a Roche Cobas 6000 c501. The correlation between the assays was assessed using Spearman's Rho correlation coefficient and the intermediate imprecision of both assays was calculated.Results: We found a strong correlation coefficient of 0.887 between FC measured on IBDoc® and the laboratory assay BÜHLMANN fCAL® turbo. The coefficients of variation (CVs) at three different FC levels were in the range 2.3-5.5% (BÜHLMANN fCAL® turbo) and in the range of 4.8-26.6% (IBDoc®).Conclusions: This study suggests that IBDoc® is a suitable alternative for the assessment of disease activity in IBD patients.

KW - Journal Article

U2 - 10.1016/j.plabm.2017.11.002

DO - 10.1016/j.plabm.2017.11.002

M3 - Journal article

C2 - 29234707

SN - 2352-5517

VL - 10

SP - 10

EP - 14

JO - Practical Laboratory Medicine

JF - Practical Laboratory Medicine

ER -

Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis

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