Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Plasmodium falciparum avoids change in erythrocytic surface expression of phagocytosis markers during inhibition of nitric oxide synthase activity

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Adjunctive dabigatran therapy improves outcome of experimental left-sided Staphylococcus aureus endocarditis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The inflamed sputum in lower respiratory tract infection: l-lactate levels are correlated to neutrophil accumulation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Antibiotic prescribing in paediatric inpatients in Ghana: a multi-centre point prevalence survey

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Nitric oxide (NO) accumulates in Plasmodium falciparum-infected erythrocytes. It may be produced by a parasite NO synthase (NOS) or by nitrate reduction. The parasite's benefit of NO accumulation is not understood. We investigated if inhibiting the P. falciparum NOS with specific and unspecific NOS inhibitors led to a decrease in intraerythrocytic NO accumulation and if this was associated with a change in surface expression of the phagocytosis markers CD47 and phosphatidyl serine. The specific inducible NOS inhibitors l-canavanine and GW274150 dose-dependently decreased intraerythrocytic NO while l-NMMA (an unspecific NOS inhibitor) and caveolin-1 scaffolding domain peptide (a specific endothelial NOS inhibitor) did not affect NO levels. Phosphatidyl serine externalization markedly increased upon P. falciparum infection. l-canavanine did not modify this whereas caveolin-1 scaffolding domain peptide increased the fraction of phosphatidyl serine exposing cells significantly. The infection did not change the level of expression of neither total CD47 nor its oxidized form. Unrelated to NOS inhibition, incubation with caveolin-1 scaffolding domain peptide lead to a decrease in oxidized CD47. In conclusion, the data imply that NOS inhibitors decrease NO accumulation in P. falciparum-infected erythrocytes but this does not correlate with the level of two major erythrocytic phagocytosis markers.

OriginalsprogEngelsk
TidsskriftMolecular and Biochemical Parasitology
Vol/bind198
Udgave nummer1
Sider (fra-til)29-36
Antal sider8
ISSN0166-6851
DOI
StatusUdgivet - nov. 2014

ID: 44827936