TY - JOUR
T1 - Plasma Small Extracellular Vesicle Cardiac miRNA Expression in Patients with Ischemic Heart Failure, Randomized to Percutaneous Intramyocardial Treatment of Adipose Derived Stem Cells or Placebo
T2 - Subanalysis of the SCIENCE Study
AU - Traxler, Denise
AU - Dannenberg, Varius
AU - Zlabinger, Katrin
AU - Gugerell, Alfred
AU - Mester-Tonczar, Julia
AU - Lukovic, Dominika
AU - Spannbauer, Andreas
AU - Hasimbegovic, Ena
AU - Kastrup, Jens
AU - Gyöngyösi, Mariann
PY - 2023/6/26
Y1 - 2023/6/26
N2 - Small extracellular vesicles (EVs) and their cargo are an important component of cell-to-cell communication in cardiac disease. Allogeneic adipose derived stem cells (ADSCs) are thought to be a potential approach for cardiac regenerative therapy in ischemic heart disease. The SCIENCE study investigated the effect of ADSCs administered via intramyocardial injection on cardiac function in patients with ischemic heart disease. The aim of this substudy, based on samples from 15 patients, was to explore small EV miRNA dynamics after treatment with ADSCs compared to a placebo. Small EVs were isolated at several timepoints after the percutaneous intramyocardial application of ADSCs. No significant effect of ADSC treatment on small EV concentration was detected. After 12 months, the expression of miR-126 decreased significantly in ADSC patients, but not in the placebo-treated group. However, all cardiac miRNAs correlated with plasma cardiac biomarkers. In line with the overall negative results of the SCIENCE study, with the exception of one miR, we did not detect any significant regulation of small EV miRNAs in this patient collective.
AB - Small extracellular vesicles (EVs) and their cargo are an important component of cell-to-cell communication in cardiac disease. Allogeneic adipose derived stem cells (ADSCs) are thought to be a potential approach for cardiac regenerative therapy in ischemic heart disease. The SCIENCE study investigated the effect of ADSCs administered via intramyocardial injection on cardiac function in patients with ischemic heart disease. The aim of this substudy, based on samples from 15 patients, was to explore small EV miRNA dynamics after treatment with ADSCs compared to a placebo. Small EVs were isolated at several timepoints after the percutaneous intramyocardial application of ADSCs. No significant effect of ADSC treatment on small EV concentration was detected. After 12 months, the expression of miR-126 decreased significantly in ADSC patients, but not in the placebo-treated group. However, all cardiac miRNAs correlated with plasma cardiac biomarkers. In line with the overall negative results of the SCIENCE study, with the exception of one miR, we did not detect any significant regulation of small EV miRNAs in this patient collective.
KW - Humans
KW - MicroRNAs/genetics
KW - Adipose Tissue
KW - Heart Failure
KW - Extracellular Vesicles/genetics
KW - Stem Cells
KW - Myocardial Ischemia/genetics
UR - http://www.scopus.com/inward/record.url?scp=85164845678&partnerID=8YFLogxK
U2 - 10.3390/ijms241310647
DO - 10.3390/ijms241310647
M3 - Journal article
C2 - 37445825
SN - 1661-6596
VL - 24
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 13
M1 - 10647
ER -