Phage-display libraries of murine and human antibody Fab fragments

J Engberg; P S Andersen; L K Nielsen; M Dziegiel; L K Johansen; B Albrechtsen

doi:10.1007/BF02761708

Phage-display libraries of murine and human antibody Fab fragments

J Engberg, P S Andersen, L K Nielsen, M Dziegiel, L K Johansen, B Albrechtsen

39 Citationer (Scopus)

Abstrakt

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

Originalsprog	Engelsk
Tidsskrift	Molecular Biotechnology
Vol/bind	6
Udgave nummer	3
Sider (fra-til)	287-310
Antal sider	24
ISSN	1073-6085
DOI	https://doi.org/10.1007/BF02761708
Status	Udgivet - dec. 1996

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10.1007/BF02761708

Citationsformater

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title = "Phage-display libraries of murine and human antibody Fab fragments",

abstract = "We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.",

keywords = "Animals, Bacteriophages, Gene Library, Humans, Immunoglobulin Fab Fragments, Mice",

author = "J Engberg and Andersen, {P S} and Nielsen, {L K} and M Dziegiel and Johansen, {L K} and B Albrechtsen",

year = "1996",

month = dec,

doi = "10.1007/BF02761708",

language = "English",

volume = "6",

pages = "287--310",

journal = "Applied Biochemistry and Biotechnology",

issn = "1073-6085",

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TY - JOUR

T1 - Phage-display libraries of murine and human antibody Fab fragments

AU - Engberg, J

AU - Andersen, P S

AU - Nielsen, L K

AU - Dziegiel, M

AU - Johansen, L K

AU - Albrechtsen, B

PY - 1996/12

Y1 - 1996/12

N2 - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

AB - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

KW - Animals

KW - Bacteriophages

KW - Gene Library

KW - Humans

KW - Immunoglobulin Fab Fragments

KW - Mice

U2 - 10.1007/BF02761708

DO - 10.1007/BF02761708

M3 - Journal article

C2 - 9067975

VL - 6

SP - 287

EP - 310

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 1073-6085

IS - 3

ER -

Phage-display libraries of murine and human antibody Fab fragments

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