Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Personalized B cell response to the Lactobacillus rhamnosus GG probiotic in healthy human subjects: a randomized trial

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

Bornholdt, J, Broholm, C, Chen, Y, Rago, A, Sloth, S, Hendel, J, Melsæther, C, Müller, CV, Juul Nielsen, M, Strickertsson, J, Engelholm, L, Vitting-Seerup, K, Jensen, KB, Baker, A & Sandelin, A 2020, 'Personalized B cell response to the Lactobacillus rhamnosus GG probiotic in healthy human subjects: a randomized trial', Gut Microbes, bind 12, nr. 1, s. 1-14. https://doi.org/10.1080/19490976.2020.1854639

APA

Bornholdt, J., Broholm, C., Chen, Y., Rago, A., Sloth, S., Hendel, J., Melsæther, C., Müller, C. V., Juul Nielsen, M., Strickertsson, J., Engelholm, L., Vitting-Seerup, K., Jensen, K. B., Baker, A., & Sandelin, A. (2020). Personalized B cell response to the Lactobacillus rhamnosus GG probiotic in healthy human subjects: a randomized trial. Gut Microbes, 12(1), 1-14. https://doi.org/10.1080/19490976.2020.1854639

CBE

Bornholdt J, Broholm C, Chen Y, Rago A, Sloth S, Hendel J, Melsæther C, Müller CV, Juul Nielsen M, Strickertsson J, Engelholm L, Vitting-Seerup K, Jensen KB, Baker A, Sandelin A. 2020. Personalized B cell response to the Lactobacillus rhamnosus GG probiotic in healthy human subjects: a randomized trial. Gut Microbes. 12(1):1-14. https://doi.org/10.1080/19490976.2020.1854639

MLA

Vancouver

Author

Bornholdt, Jette ; Broholm, Christa ; Chen, Yun ; Rago, Alfredo ; Sloth, Stine ; Hendel, Jakob ; Melsæther, Cathrine ; Müller, Christina V ; Juul Nielsen, Maria ; Strickertsson, Jesper ; Engelholm, Lars ; Vitting-Seerup, Kristoffer ; Jensen, Kim B ; Baker, Adam ; Sandelin, Albin. / Personalized B cell response to the Lactobacillus rhamnosus GG probiotic in healthy human subjects : a randomized trial. I: Gut Microbes. 2020 ; Bind 12, Nr. 1. s. 1-14.

Bibtex

@article{f529c3298be9490f89eca3250852b0e3,
title = "Personalized B cell response to the Lactobacillus rhamnosus GG probiotic in healthy human subjects: a randomized trial",
abstract = "The specific effects of administering live probiotics in the human gut are not well characterized. To this end, we investigated the immediate effect of Lactobacillus rhamnosus GG (LGG) in the jejunum of 27 healthy volunteers 2 h after ingestion using a combination of global RNA sequencing of human biopsies and bacterial DNA sequencing in a multi-visit, randomized, cross-over design (ClinicalTrials.gov number NCT03140878). While LGG was detectable in jejunum after 2 h in treated subjects, the gene expression response vs. placebo was subtle if assessed across all subjects. However, clustering analysis revealed that one-third of subjects exhibited a strong and consistent LGG response involving hundreds of genes, where genes related to B cell activation were upregulated, consistent with prior results in mice. Immunohistochemistry and single cell-based deconvolution analyses showed that this B cell signature likely is due to activation and proliferation of existing B cells rather than B cell immigration to the tissue. Our results indicate that the LGG strain has an immediate effect in the human gut in a subpopulation of individuals. In extension, our data strongly suggest that studies on in vivo probiotic effects in humans require large cohorts and must take individual variation into account.",
keywords = "B cell activation, human transcriptomics, immediate in vivo effect, Lactobacillus rhamnosus GG, probiotics",
author = "Jette Bornholdt and Christa Broholm and Yun Chen and Alfredo Rago and Stine Sloth and Jakob Hendel and Cathrine Mels{\ae}ther and M{\"u}ller, {Christina V} and {Juul Nielsen}, Maria and Jesper Strickertsson and Lars Engelholm and Kristoffer Vitting-Seerup and Jensen, {Kim B} and Adam Baker and Albin Sandelin",
year = "2020",
month = nov,
day = "9",
doi = "10.1080/19490976.2020.1854639",
language = "English",
volume = "12",
pages = "1--14",
journal = "Gut Microbes",
issn = "1949-0976",
publisher = "Landes Bioscience",
number = "1",

}

RIS

TY - JOUR

T1 - Personalized B cell response to the Lactobacillus rhamnosus GG probiotic in healthy human subjects

T2 - a randomized trial

AU - Bornholdt, Jette

AU - Broholm, Christa

AU - Chen, Yun

AU - Rago, Alfredo

AU - Sloth, Stine

AU - Hendel, Jakob

AU - Melsæther, Cathrine

AU - Müller, Christina V

AU - Juul Nielsen, Maria

AU - Strickertsson, Jesper

AU - Engelholm, Lars

AU - Vitting-Seerup, Kristoffer

AU - Jensen, Kim B

AU - Baker, Adam

AU - Sandelin, Albin

PY - 2020/11/9

Y1 - 2020/11/9

N2 - The specific effects of administering live probiotics in the human gut are not well characterized. To this end, we investigated the immediate effect of Lactobacillus rhamnosus GG (LGG) in the jejunum of 27 healthy volunteers 2 h after ingestion using a combination of global RNA sequencing of human biopsies and bacterial DNA sequencing in a multi-visit, randomized, cross-over design (ClinicalTrials.gov number NCT03140878). While LGG was detectable in jejunum after 2 h in treated subjects, the gene expression response vs. placebo was subtle if assessed across all subjects. However, clustering analysis revealed that one-third of subjects exhibited a strong and consistent LGG response involving hundreds of genes, where genes related to B cell activation were upregulated, consistent with prior results in mice. Immunohistochemistry and single cell-based deconvolution analyses showed that this B cell signature likely is due to activation and proliferation of existing B cells rather than B cell immigration to the tissue. Our results indicate that the LGG strain has an immediate effect in the human gut in a subpopulation of individuals. In extension, our data strongly suggest that studies on in vivo probiotic effects in humans require large cohorts and must take individual variation into account.

AB - The specific effects of administering live probiotics in the human gut are not well characterized. To this end, we investigated the immediate effect of Lactobacillus rhamnosus GG (LGG) in the jejunum of 27 healthy volunteers 2 h after ingestion using a combination of global RNA sequencing of human biopsies and bacterial DNA sequencing in a multi-visit, randomized, cross-over design (ClinicalTrials.gov number NCT03140878). While LGG was detectable in jejunum after 2 h in treated subjects, the gene expression response vs. placebo was subtle if assessed across all subjects. However, clustering analysis revealed that one-third of subjects exhibited a strong and consistent LGG response involving hundreds of genes, where genes related to B cell activation were upregulated, consistent with prior results in mice. Immunohistochemistry and single cell-based deconvolution analyses showed that this B cell signature likely is due to activation and proliferation of existing B cells rather than B cell immigration to the tissue. Our results indicate that the LGG strain has an immediate effect in the human gut in a subpopulation of individuals. In extension, our data strongly suggest that studies on in vivo probiotic effects in humans require large cohorts and must take individual variation into account.

KW - B cell activation

KW - human transcriptomics

KW - immediate in vivo effect

KW - Lactobacillus rhamnosus GG

KW - probiotics

U2 - 10.1080/19490976.2020.1854639

DO - 10.1080/19490976.2020.1854639

M3 - Journal article

C2 - 33274667

VL - 12

SP - 1

EP - 14

JO - Gut Microbes

JF - Gut Microbes

SN - 1949-0976

IS - 1

ER -

ID: 61525216