TY - JOUR
T1 - Penicillin reduces eustachian tube gland tissue changes in acute otitis media
AU - Andersen, Henrik
AU - Thomsen, Jens
AU - Cayé-Thomasen, Per
PY - 2005/8
Y1 - 2005/8
N2 - OBJECTIVE: The volume of the mucous paratubal glands and the number of the mucus-producing goblet cells in the middle ear and Eustachian tube (ET) are increased after experimental acute otitis media (AOM). The present investigation examines a potential effect of penicillin on the changes in goblet cell density and gland structures of the ET during and after AOM.STUDY DESIGN: Middle ear inoculation of Streptococcus pneumoniae in 50 rats. Two days later, 25 rats were given penicillin V as one daily dose for 5 days. Twenty-five rats received no treatment. Five animals from each group were sacrificed on days 4, 8, 16, 90, and 180. The ET was dissected and decalcified, followed by paraffin embedding, serial transverse sectioning, and PAS/alcian blue staining. The goblet cell density and the paratubal gland composition and volume were determined in every 20th section, using a light microscope.RESULTS: Penicillin reduced the increase of goblet cell density from day 8 and through 6 months, whereas the increase of the paratubal mucous gland volume was unaffected by treatment.CONCLUSION: We conclude that penicillin reduces the increase of ET goblet cell density during and after acute otitis media, whereas the paratubal gland volume remains unaffected. An increased mucosal secretory capacity and indicated excessive secretion of mucus may contribute to the deteriorated ET function found after AOM and thus predispose, sustain, or aggravate middle ear disease. This may be prevented by penicillin treatment.
AB - OBJECTIVE: The volume of the mucous paratubal glands and the number of the mucus-producing goblet cells in the middle ear and Eustachian tube (ET) are increased after experimental acute otitis media (AOM). The present investigation examines a potential effect of penicillin on the changes in goblet cell density and gland structures of the ET during and after AOM.STUDY DESIGN: Middle ear inoculation of Streptococcus pneumoniae in 50 rats. Two days later, 25 rats were given penicillin V as one daily dose for 5 days. Twenty-five rats received no treatment. Five animals from each group were sacrificed on days 4, 8, 16, 90, and 180. The ET was dissected and decalcified, followed by paraffin embedding, serial transverse sectioning, and PAS/alcian blue staining. The goblet cell density and the paratubal gland composition and volume were determined in every 20th section, using a light microscope.RESULTS: Penicillin reduced the increase of goblet cell density from day 8 and through 6 months, whereas the increase of the paratubal mucous gland volume was unaffected by treatment.CONCLUSION: We conclude that penicillin reduces the increase of ET goblet cell density during and after acute otitis media, whereas the paratubal gland volume remains unaffected. An increased mucosal secretory capacity and indicated excessive secretion of mucus may contribute to the deteriorated ET function found after AOM and thus predispose, sustain, or aggravate middle ear disease. This may be prevented by penicillin treatment.
KW - Acute Disease
KW - Animals
KW - Biopsy, Needle
KW - Disease Models, Animal
KW - Down-Regulation
KW - Eustachian Tube/drug effects
KW - Goblet Cells/drug effects
KW - Immunohistochemistry
KW - Male
KW - Microscopy, Electron
KW - Otitis Media/drug therapy
KW - Otolaryngology/methods
KW - Penicillins/pharmacology
KW - Random Allocation
KW - Rats
KW - Rats, Sprague-Dawley
KW - Sensitivity and Specificity
U2 - 10.1016/j.otohns.2005.02.020
DO - 10.1016/j.otohns.2005.02.020
M3 - Journal article
C2 - 16087016
SN - 0194-5998
VL - 133
SP - 206
EP - 210
JO - Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
JF - Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
IS - 2
ER -