@article{7877640e2f7a45b4a73a5f4c86338f65,
title = "Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes",
abstract = "This study investigated the effects of strict glycaemic control on beta-cell function in nine obese subjects with type 2 diabetes (T2DM), using graded glucose infusions together with infusions of saline or GLP-1 before (HbA(1)c: 8.0+/-0.4%) and after four weeks of near-normalization of blood glucose (BG) using insulin (mean diurnal BG: 6.4+/-0.3 mmol/l; HbA(1)c: 6.6+/-0.3%). Nine matched healthy subjects acted as controls. In controls, area-under-curve (AUC) for amylin, C-peptide and proinsulin were higher with GLP-1 than saline (P<0.001). The AUC amylin/C-peptide ratio was similar on both days, while AUC proinsulin/C-peptide ratio was higher with GLP-1 (P=0.02). In the patients, amylin, C-peptide and proinsulin AUCs were unaltered by near-normoglycaemia per se. Proinsulin responses to GLP-1 were unchanged, but amylin and C-peptide AUCs increased (P<0.05) after insulin treatment, and AUC amylin/C-peptide ratios rose to control levels. Near-normoglycaemia tended to reduce AUC proinsulin/C-peptide ratio, which was significant (P=0.04) with GLP-1, but still higher than with saline (P=0.004). In conclusion, amylin, C-peptide and proinsulin responses to glucose were unaffected by four weeks of near-normoglycaemia, whereas GLP-1 increased amylin and C-peptide secretion and amylin/C-peptide ratio. Near-normoglycaemia reduced proinsulin/C-peptide ratio during stimulation with GLP-1, suggesting that strict glycaemic control might ameliorate some of the disturbances in beta-cell function characterizing T2DM.",
keywords = "Amyloid, Area Under Curve, Blood Glucose, C-Peptide, Diabetes Mellitus, Type 2, Female, Glucagon-Like Peptide 1, Glucose, Humans, Incretins, Insulin, Insulin-Secreting Cells, Male, Middle Aged, Proinsulin, Time Factors",
author = "Meena Asmar and H{\o}jberg, {Patricia V} and Deacon, {Carolyn F} and Kristine Hare and Holst, {Jens J} and Sten Madsbad",
note = "Copyright 2010 Elsevier B.V. All rights reserved.",
year = "2010",
doi = "10.1016/j.regpep.2009.12.004",
language = "English",
volume = "160",
pages = "175--80",
journal = "Regulatory Peptides",
issn = "1873-1686",
publisher = "Elsevier",
number = "1-3",
}