Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

P. aeruginosa flow-cell biofilms are enhanced by repeated phage treatments but can be eradicated by phage-ciprofloxacin combination

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Defective interferon priming and impaired antiviral responses in a patient with an IRF7 variant and severe influenza

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Pandemics: past, present, future: That is like choosing between cholera and plague

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  2. Anti-biofilm Approach in Infective Endocarditis Exposes New Treatment Strategies for Improved Outcome

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. Adjunctive S100A8/A9 Immunomodulation Hinders Ciprofloxacin Resistance in Pseudomonas aeruginosa in a Murine Biofilm Wound Model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Autologous fibrin sealant co-delivered with antibiotics is a robust method for topical antibiotic treatment after sinus surgery

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Karoline Henriksen
  • Nanna Rørbo
  • Morten Levin Rybtke
  • Mark Grevsen Martinet
  • Tim Tolker-Nielsen
  • Niels Høiby
  • Mathias Middelboe
  • Oana Ciofu
Vis graf over relationer

Phage therapy has shown promising results in the treatment of Pseudomonas aeruginosa biofilm infections in animal studies and case reports. The aim of this study was to quantify effects of phage treatments on P. aeruginosa biofilm production and structure. Confocal scanning microscopy was used to follow the interaction between a cocktail of three virulent phages and P. aeruginosa flow-cell biofilms. The role of (i) biofilm age, (ii) repeated phage treatments, (iii) alginate production and (iv) the combination with sub-MIC levels of ciprofloxacin was investigated. Single phage treatment in the early biofilm stages significantly reduced P. aeruginosa PAO1 biovolume (85%-98% reduction). Repeated phage treatments increased the biovolume from 18.25 (untreated biofilm) to 22.24 and 31.07 µm3/µm2 for biofilms treated with phages twice and thrice, respectively. Alginate protected against the phage treatment as the live biovolume remained unaffected by the phage treatment in the mucoid biofilm (20.11 µm3/µm2 in untreated and 21.74 µm3/µm2 in phage-treated biofilm) but decreased in the PAO1 biofilm from 27.35 to 0.89 µm3/µm2. We show that the combination of phages with antibiotics at sub-MIC levels caused a ∼6 log units reduction in the abundance of P. aeruginosa cells in biofilms and that phage treatment increased the size of microcolonies in flow-cell system.

OriginalsprogEngelsk
TidsskriftMedical Microbiology and Immunology
Vol/bind77
Udgave nummer2
Sider (fra-til)ftz011
ISSN0300-8584
DOI
StatusUdgivet - 1 mar. 2019

Bibliografisk note

© FEMS 2019.

ID: 59045599