Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Optimizing combination of vascular endothelial growth factor and mesenchymal stem cells on ectopic bone formation in SCID mice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Effect of nanocoating with rhamnogalacturonan-I on surface properties and osteoblasts response

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Cell culture plastics with immobilized interleukin-4 for monocyte differentiation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Decreased markers of bone turnover in children and adolescents with type 1 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Bone resorption is unchanged by liraglutide in type 2 diabetes patients: A randomised controlled trial

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Bone turnover markers during the remission phase in children and adolescents with type 1 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

INTRODUCTION: Insufficient blood supply may limit bone regeneration in bone defects. Vascular endothelial growth factor (VEGF) promotes angiogenesis by increasing endothelial migration. This outcome, however, could depend on time of application. Sheep mesenchymal stem cells (MSCs) in severe combined immunodeficient (SCID) mice were used in this study to evaluate optimal time points for VEGF stimulation to increase bone formation.

METHODS: Twenty-eight SCID (NOD.CB17-Prkdcscid /J) mice had hydroxyapatite granules seeded with 5 × 105 MSCs inserted subcutaneous. Pellets released VEGF on days 1-7, days 1-14, days 1-21, days 1-42, days 7-14, and days 21-42. After 8 weeks, the implant-bone-blocks were harvested, paraffin embedded, sectioned, and stained with both hematoxylin and eosin (HE) and immunohistochemistry for human vimentin (hVim) staining. Blood samples were collected for determination of bone-related biomarkers in serum.

RESULTS: The groups with 5 × 105 MSCs and VEGF stimulation on days 1-14 and days 1-21 showed more bone formation when compared to the control group of 5 × 105 MSCs alone (p < 0.01). Serum biomarkers had no significant values. The hVim staining confirmed the ovine origin of the observed ectopic bone formation.

CONCLUSION: Optimal bone formation of MSCs was reached when stimulating with VEGF during the first 14 or 21 days after surgery. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3326-3332, 2017.

OriginalsprogEngelsk
TidsskriftJournal of Biomedical Materials Research - Part A
Vol/bind105
Udgave nummer12
Sider (fra-til)3326-3332
Antal sider7
ISSN1549-3296
DOI
StatusUdgivet - dec. 2017

ID: 52109442