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Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells

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Sand, Nicolai T ; Petersen, Tobias B ; Bang-Christensen, Sara R ; Ahrens, Theresa D ; Løppke, Caroline ; Jørgensen, Amalie M ; Gustavsson, Tobias ; Choudhary, Swati ; Theander, Thor G ; Salanti, Ali ; Agerbæk, Mette Ø. / Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells. I: International Journal of Molecular Sciences. 2020 ; Bind 21, Nr. 7.

Bibtex

@article{b6595f176f8b458f9bc096eeb4bbfcd9,
title = "Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells",
abstract = "Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.",
keywords = "Antigens, Protozoan/genetics, Cell Line, Tumor, Chondroitin Sulfates/metabolism, Early Detection of Cancer/methods, Humans, Magnetics, Neoplastic Cells, Circulating/pathology, Recombinant Proteins",
author = "Sand, {Nicolai T} and Petersen, {Tobias B} and Bang-Christensen, {Sara R} and Ahrens, {Theresa D} and Caroline L{\o}ppke and J{\o}rgensen, {Amalie M} and Tobias Gustavsson and Swati Choudhary and Theander, {Thor G} and Ali Salanti and Agerb{\ae}k, {Mette {\O}}",
year = "2020",
month = mar,
day = "31",
doi = "10.3390/ijms21072401",
language = "English",
volume = "21",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Molecular Diversity Preservation International (M D P I)",
number = "7",

}

RIS

TY - JOUR

T1 - Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells

AU - Sand, Nicolai T

AU - Petersen, Tobias B

AU - Bang-Christensen, Sara R

AU - Ahrens, Theresa D

AU - Løppke, Caroline

AU - Jørgensen, Amalie M

AU - Gustavsson, Tobias

AU - Choudhary, Swati

AU - Theander, Thor G

AU - Salanti, Ali

AU - Agerbæk, Mette Ø

PY - 2020/3/31

Y1 - 2020/3/31

N2 - Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.

AB - Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.

KW - Antigens, Protozoan/genetics

KW - Cell Line, Tumor

KW - Chondroitin Sulfates/metabolism

KW - Early Detection of Cancer/methods

KW - Humans

KW - Magnetics

KW - Neoplastic Cells, Circulating/pathology

KW - Recombinant Proteins

U2 - 10.3390/ijms21072401

DO - 10.3390/ijms21072401

M3 - Journal article

C2 - 32244341

VL - 21

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 7

ER -

ID: 62343700