Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Omics-based tracking of Pseudomonas aeruginosa persistence in "eradicated" cystic fibrosis patients.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. COVID-19: guidance on palliative care from a European Respiratory Society international task force

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Morbidity and mortality in carriers of the cystic fibrosis mutation CFTR Phe508del in the general population

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. The DisEntangling Chronic Obstructive pulmonary Disease Exacerbations clinical trials NETwork (DECODE-NET): rationale and vision

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Clinical considerations in individuals with α1-antitrypsin PI*SZ genotype

    Publikation: Bidrag til tidsskriftReviewpeer review

  5. The effect of exacerbation history on outcomes in the IMPACT trial

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Whenever Pseudomonas aeruginosa is cultured from cystic fibrosis (CF) patient airways, the primary goal is eradication by antibiotic therapy. Success is defined by ≥6 months of negative bacterial airway cultures. However, we suspect that P. aeruginosa persists in airways without clinical detection for long periods.Out of 298 P. aeruginosa-infected Copenhagen CF patients, we identified 80 with complete P. aeruginosa monitoring records and measured their maximum P. aeruginosa-free eradication periods (MEP). Isolates from 72 patients were whole-genome sequenced (n=567) and clone typed. Select isolate relatedness was examined through phylogenetic analysis and phenotypic multivariate modelling.69 (86%) patients exhibited eradication in the monitoring period (2002-2018). Sequenced isolates bridged the MEP of 42 patients, and the same clone type persisted over the MEP in 18 (43%) patients. Patients with failed eradication were on average treated more intensively with antibiotics, but this may be linked to their more severe pre-MEP infection trajectories. Of the 42 patients, 26 also had sinus surgery; the majority (n=15) showed MEPs adjacent to surgery, and only five had persisting clone types. Importantly, combined phylogenetic-phenomic evaluation suggests that persisting clone types are a result of re-emergence of the same strain rather than re-infection from the environment, and similar relatedness is exhibited by paired lower and upper airway samples and in transmission cases.In conclusion, nearly half of CF patients with supposed eradication may not truly be cleared of their original bacteria according to omics-based monitoring. This distinct cohort that is persistently infected would probably benefit from tailored antibiotic therapy.

OriginalsprogEngelsk
TidsskriftThe European respiratory journal
Vol/bind57
Udgave nummer4
ISSN0903-1936
DOI
StatusUdgivet - apr. 2021

ID: 62033538