Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Olfactomedin 4 defines a subset of human neutrophils

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Clemmensen, Stine N ; Bohr, Christina T. ; Rørvig, Sara ; Glenthøj, Andreas ; Mora-Jensen, Helena Isabel ; Cramer, Elisabeth P ; Jacobsen, Lars C ; Larsen, Maria T ; Cowland, Jack B ; Tanassi, Julia T ; Heegaard, Niels Henrik Helweg ; Wren, Jonathan D ; Silahtaroglu, Asli ; Borregaard, Niels. / Olfactomedin 4 defines a subset of human neutrophils. I: Journal of Leukocyte Biology. 2012 ; Bind 91, Nr. 3. s. 495-500.

Bibtex

@article{54025bf8659f45608530b4a2003d0654,
title = "Olfactomedin 4 defines a subset of human neutrophils",
abstract = "OLFM4 was identified initially as a gene highly induced in myeloid stem cells by G-CSF treatment. A bioinformatics method using a global meta-analysis of microarray data predicted that OLFM4 would be associated with specific granules in human neutrophils. Subcellular fractionation of peripheral blood neutrophils demonstrated complete colocalization of OLFM4 with the specific granule protein NGAL, and stimulation of neutrophils with PMA resulted in corelease of NGAL and OLFM4, proving that OLFM4 is a genuine constituent of neutrophil-specific granules. In accordance with this, OLFM4 mRNA peaked at the MY/MM stage of maturation. OLFM4 was, however, present in only 20-25{\%} of peripheral blood neutrophils, as determined by immunocytochemistry and flow cytometry, whereas mRNA for OLFM4 was present in all MY/MM, indicating post-transcriptional regulation as a basis for the heterogeneous expression of OLFM4 protein.",
author = "Clemmensen, {Stine N} and Bohr, {Christina T.} and Sara R{\o}rvig and Andreas Glenth{\o}j and Mora-Jensen, {Helena Isabel} and Cramer, {Elisabeth P} and Jacobsen, {Lars C} and Larsen, {Maria T} and Cowland, {Jack B} and Tanassi, {Julia T} and Heegaard, {Niels Henrik Helweg} and Wren, {Jonathan D} and Asli Silahtaroglu and Niels Borregaard",
year = "2012",
doi = "10.1189/jlb.0811417",
language = "English",
volume = "91",
pages = "495--500",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "Federation of American Societies for Experimental Biology",
number = "3",

}

RIS

TY - JOUR

T1 - Olfactomedin 4 defines a subset of human neutrophils

AU - Clemmensen, Stine N

AU - Bohr, Christina T.

AU - Rørvig, Sara

AU - Glenthøj, Andreas

AU - Mora-Jensen, Helena Isabel

AU - Cramer, Elisabeth P

AU - Jacobsen, Lars C

AU - Larsen, Maria T

AU - Cowland, Jack B

AU - Tanassi, Julia T

AU - Heegaard, Niels Henrik Helweg

AU - Wren, Jonathan D

AU - Silahtaroglu, Asli

AU - Borregaard, Niels

PY - 2012

Y1 - 2012

N2 - OLFM4 was identified initially as a gene highly induced in myeloid stem cells by G-CSF treatment. A bioinformatics method using a global meta-analysis of microarray data predicted that OLFM4 would be associated with specific granules in human neutrophils. Subcellular fractionation of peripheral blood neutrophils demonstrated complete colocalization of OLFM4 with the specific granule protein NGAL, and stimulation of neutrophils with PMA resulted in corelease of NGAL and OLFM4, proving that OLFM4 is a genuine constituent of neutrophil-specific granules. In accordance with this, OLFM4 mRNA peaked at the MY/MM stage of maturation. OLFM4 was, however, present in only 20-25% of peripheral blood neutrophils, as determined by immunocytochemistry and flow cytometry, whereas mRNA for OLFM4 was present in all MY/MM, indicating post-transcriptional regulation as a basis for the heterogeneous expression of OLFM4 protein.

AB - OLFM4 was identified initially as a gene highly induced in myeloid stem cells by G-CSF treatment. A bioinformatics method using a global meta-analysis of microarray data predicted that OLFM4 would be associated with specific granules in human neutrophils. Subcellular fractionation of peripheral blood neutrophils demonstrated complete colocalization of OLFM4 with the specific granule protein NGAL, and stimulation of neutrophils with PMA resulted in corelease of NGAL and OLFM4, proving that OLFM4 is a genuine constituent of neutrophil-specific granules. In accordance with this, OLFM4 mRNA peaked at the MY/MM stage of maturation. OLFM4 was, however, present in only 20-25% of peripheral blood neutrophils, as determined by immunocytochemistry and flow cytometry, whereas mRNA for OLFM4 was present in all MY/MM, indicating post-transcriptional regulation as a basis for the heterogeneous expression of OLFM4 protein.

U2 - 10.1189/jlb.0811417

DO - 10.1189/jlb.0811417

M3 - Journal article

VL - 91

SP - 495

EP - 500

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 3

ER -

ID: 33231152