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Ocular surface microbiota in patients with aqueous tear-deficient dry eye

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Andersson, Jasmine ; Vogt, Josef K ; Dalgaard, Marlene D ; Pedersen, Oluf ; Holmgaard, Kim ; Heegaard, Steffen. / Ocular surface microbiota in patients with aqueous tear-deficient dry eye. I: The ocular surface. 2021 ; Bind 19. s. 210-217.

Bibtex

@article{5b5e9d3d47eb4a6bb43f4d2a0f1e1174,
title = "Ocular surface microbiota in patients with aqueous tear-deficient dry eye",
abstract = "PURPOSE: An altered ocular surface microbiota may contribute to the pathophysiology of dry eye disease. The aim of the study was to explore potential differences in microbiota diversity and composition in aqueous tear-deficient dry eye (with and without ocular graft-versus-host disease) compared with controls.METHODS: Swab samples from the inferior fornix of the conjunctiva were obtained from patients with aqueous tear-deficient dry eye with and without ocular graft-versus-host disease (n = 18, n = 21, respectively) and controls (n = 28). Isolated bacterial DNA from swabs were analyzed with 16S rRNA gene amplicon sequencing.RESULTS: Decreased microbiota diversity was observed in patients with aqueous tear-deficient dry eye (p ≤ 0.003) who also showed a difference in microbiota composition compared with controls (p = 0.001). Although several genera were less abundant in aqueous tear-deficient dry eye, a minimal core ocular surface microbiota comprising five genera was shared by >75% of the study participants: Enhydrobacter, Brevibacterium, Staphylococcus, Streptococcus and Cutibacterium. Pseudomonas was identified as a bacterial biomarker for controls and Bacilli for patients with aqueous tear-deficient dry eye.CONCLUSIONS: Ocular surface microbiota in patients with aqueous tear-deficient dry eye was characterized by an aberrant microbiota composition in comparison to controls, with decreased diversity and reduced relative abundances of several genera. Additionally, a few genera were present in most of the study population, indicating that a minimal core ocular surface microbiota may exist.",
keywords = "16S rRNA gene amplicon sequencing, ADDE, Bacilli class, Bacterial biomarker, Dry eye, Ocular surface microbiota",
author = "Jasmine Andersson and Vogt, {Josef K} and Dalgaard, {Marlene D} and Oluf Pedersen and Kim Holmgaard and Steffen Heegaard",
note = "Copyright {\textcopyright} 2020 Elsevier Inc. All rights reserved.",
year = "2021",
month = jan,
doi = "10.1016/j.jtos.2020.09.003",
language = "English",
volume = "19",
pages = "210--217",
journal = "Ocular Surface",
issn = "1542-0124",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Ocular surface microbiota in patients with aqueous tear-deficient dry eye

AU - Andersson, Jasmine

AU - Vogt, Josef K

AU - Dalgaard, Marlene D

AU - Pedersen, Oluf

AU - Holmgaard, Kim

AU - Heegaard, Steffen

N1 - Copyright © 2020 Elsevier Inc. All rights reserved.

PY - 2021/1

Y1 - 2021/1

N2 - PURPOSE: An altered ocular surface microbiota may contribute to the pathophysiology of dry eye disease. The aim of the study was to explore potential differences in microbiota diversity and composition in aqueous tear-deficient dry eye (with and without ocular graft-versus-host disease) compared with controls.METHODS: Swab samples from the inferior fornix of the conjunctiva were obtained from patients with aqueous tear-deficient dry eye with and without ocular graft-versus-host disease (n = 18, n = 21, respectively) and controls (n = 28). Isolated bacterial DNA from swabs were analyzed with 16S rRNA gene amplicon sequencing.RESULTS: Decreased microbiota diversity was observed in patients with aqueous tear-deficient dry eye (p ≤ 0.003) who also showed a difference in microbiota composition compared with controls (p = 0.001). Although several genera were less abundant in aqueous tear-deficient dry eye, a minimal core ocular surface microbiota comprising five genera was shared by >75% of the study participants: Enhydrobacter, Brevibacterium, Staphylococcus, Streptococcus and Cutibacterium. Pseudomonas was identified as a bacterial biomarker for controls and Bacilli for patients with aqueous tear-deficient dry eye.CONCLUSIONS: Ocular surface microbiota in patients with aqueous tear-deficient dry eye was characterized by an aberrant microbiota composition in comparison to controls, with decreased diversity and reduced relative abundances of several genera. Additionally, a few genera were present in most of the study population, indicating that a minimal core ocular surface microbiota may exist.

AB - PURPOSE: An altered ocular surface microbiota may contribute to the pathophysiology of dry eye disease. The aim of the study was to explore potential differences in microbiota diversity and composition in aqueous tear-deficient dry eye (with and without ocular graft-versus-host disease) compared with controls.METHODS: Swab samples from the inferior fornix of the conjunctiva were obtained from patients with aqueous tear-deficient dry eye with and without ocular graft-versus-host disease (n = 18, n = 21, respectively) and controls (n = 28). Isolated bacterial DNA from swabs were analyzed with 16S rRNA gene amplicon sequencing.RESULTS: Decreased microbiota diversity was observed in patients with aqueous tear-deficient dry eye (p ≤ 0.003) who also showed a difference in microbiota composition compared with controls (p = 0.001). Although several genera were less abundant in aqueous tear-deficient dry eye, a minimal core ocular surface microbiota comprising five genera was shared by >75% of the study participants: Enhydrobacter, Brevibacterium, Staphylococcus, Streptococcus and Cutibacterium. Pseudomonas was identified as a bacterial biomarker for controls and Bacilli for patients with aqueous tear-deficient dry eye.CONCLUSIONS: Ocular surface microbiota in patients with aqueous tear-deficient dry eye was characterized by an aberrant microbiota composition in comparison to controls, with decreased diversity and reduced relative abundances of several genera. Additionally, a few genera were present in most of the study population, indicating that a minimal core ocular surface microbiota may exist.

KW - 16S rRNA gene amplicon sequencing

KW - ADDE

KW - Bacilli class

KW - Bacterial biomarker

KW - Dry eye

KW - Ocular surface microbiota

U2 - 10.1016/j.jtos.2020.09.003

DO - 10.1016/j.jtos.2020.09.003

M3 - Journal article

C2 - 32931939

VL - 19

SP - 210

EP - 217

JO - Ocular Surface

JF - Ocular Surface

SN - 1542-0124

ER -

ID: 61960965