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Novel porcine model of implant-associated osteomyelitis: A comprehensive analysis of local, regional, and systemic response

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  • Louise Kruse Jensen
  • Janne Koch
  • Kirstine Dich-Jorgensen
  • Bent Aalbaek
  • Andreas Petersen
  • Kurt Fuursted
  • Thomas Bjarnsholt
  • Kasper Nørskov Kragh
  • Mikkel Tøtterup
  • Mats Bue
  • Pelle Hanberg
  • Kjeld Søballe
  • Peter M H Heegaard
  • Henrik Elvang Jensen
Vis graf over relationer

Pigs are favorable experimental animals for infectious diseases in humans. However, implant-associated osteomyelitis (IAO) models in pigs have only been evaluated using high-inoculum infection (>108CFU) models in 1975 and 1993. Therefore, the aim of this paper was to present a new low inoculum porcine model of human IAO based on 42 experimental pigs. The model was created by drilling an implant cavity in the tibial bone followed by insertion of a small steel implant and simultaneous inoculation of Staphylococcus aureus bacteria (n = 32) or saline (n = 10). The infected pigs were either inoculated with 104CFU (n = 26) or 102and 103CFU (n = 6). All animals were euthanized 5 days after insertion of implants. Pigs receiving the high-inoculum infections showed a significantly higher volume of bone lesion, number of neutrophils around the implant, concentrations of acute phase proteins in serum, and enlargement of regional lymph nodes. A positive correlation was present between a high number of surrounding neutrophils and high values of all other parameters. Furthermore, a threshold of 40 neutrophils per 10 high power fields for the histopathological diagnosis of high grade IAO was defined.

IN CONCLUSION: This paper describes a novel low-inoculum S. aureus porcine model of IAO which was demonstrated to be reliable, reproducible and discriminative to human IAO, and represents a requested and valuable tool in orthopedic research. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2211-2221, 2017.

OriginalsprogEngelsk
TidsskriftJournal of orthopaedic research : official publication of the Orthopaedic Research Society
Vol/bind35
Udgave nummer10
Sider (fra-til)2211-2221
Antal sider11
ISSN0736-0266
DOI
StatusUdgivet - okt. 2017

ID: 52705874