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Novel functions of the luteinizing hormone/chorionic gonadotropin receptor in prostate cancer cells and patients

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  1. Experiences managing pregnant hospital staff members using an active management policy-A qualitative study

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  2. A Bayesian reanalysis of the effects of hydroxychloroquine and azithromycin on viral carriage in patients with COVID-19

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  3. Histological outcomes in HPV-screened elderly women in Denmark

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  4. Prevalence of MRSA nasal carriage among pregnant women in Copenhagen

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  1. The influence of prenatal exposure to phthalates on subsequent male growth and body composition in adolescence

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  2. The calcium-sensing receptor is essential for calcium and bicarbonate sensitivity in human spermatozoa

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Possible Relevance of Soluble Luteinizing Hormone Receptor during Development and Adulthood in Boys and Men

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  4. Establishment of a novel human fetal adrenal culture model that supports de novo and manipulated steroidogenesis

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  5. CPMS-improving patient care in Europe via virtual case discussions

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Vis graf over relationer

Prostate cancer (PCa) cells become castrate-resistant after initial tumor regression following castration-based lowering of testosterone (T). De-novo intra-tumoral steroid synthesis is a suggested biological mechanism of castration resistant PCa, but the regulators are unknown. Testicular T production is controlled by the luteinizing hormone/choriogonadotropin receptor (LHCGR). To elucidate the influence of LHCGR on PCa development the presence and effects of LHCGR in PCa and whether LHCGR in serum holds prognostic information in PCa patients is investigated. LHCGR expression was investigated by RT-PCR, WB, IHC, qPCR in PCa cell lines and prostatic tissue. Steroid production was measured in media from cell lines with LC-MS/MS and expression of steroidogenic enzymes with qPCR. Serum LHCGR (sLHCGR) was measured with ELISA in PCa patients (N = 157). Presence of LHCGR was established in prostatic tissue and PCa cell lines. Cell proliferation increased by 1.29-fold in LNCaP (P = 0.007) and 1.33-fold in PC-3 cells (P = 0.0007), when stimulated by luteinizing hormone. Choriogonadotropin stimulation decreased proliferation 0.93-fold in DU145 cells (P = 0.05), but none of the treatments altered steroid metabolite secretion. Low sLHCGR concentration was associated with a higher risk of biochemical failure after radical prostatectomy (HR = 3.05, P = 0.06) and castration resistance (HR = 6.92, P = 0.004) compared to high sLHCGR concentration. LHCGR is expressed in PCa and may exert a growth regulatory role in PCa derived cell lines. A potential prognostic role of sLHCGR for determining recurrence risk in PCa patients is found in this pilot study but needs verification in larger cohorts.

OriginalsprogEngelsk
Artikelnummere0238814
TidsskriftPLoS One
Vol/bind15
Udgave nummer9 September
Sider (fra-til)e0238814
ISSN1932-6203
DOI
StatusUdgivet - 3 sep. 2020

ID: 60879307