No effect of MDMA (ecstasy) on cell death and 5-HT2A receptor density in organotypic rat hippocampal cultures

Marie-Louise Sveen, Gitte M Knudsen, Susana Aznar

Abstract

MDMA (3,4 Methylenedioxy-methamphetamine) binds and blocks the presynaptic serotonin reuptake transporters and postsynaptic serotonin 5-HT2A receptors, with highest affinity for the first. Whether 5-HT2A receptor density decreases due to MDMA's direct effect on postsynaptic serotonin receptors is at present not known. This study analyzes whether direct stimulation of the postsynaptic 5-HT2A receptor by MDMA in organotypic hippocampal cultures results in cell death and downregulation of this receptor. Fifty or 100 microM MDMA was added to 1 week old cultures, made of 11 day old rat pups. Fluorojade and immunostaining for MAP2 and 5-HT2A to determine neurodegeneration, and changes in receptor density, respectively, resulted in no significant differences. MDMA's neurotoxicity and regulation of post-synaptic 5-HT2A receptors thus seems to require the presence of intact serotonergic terminals.

OriginalsprogEngelsk
TidsskriftNeuroscience Letters
Vol/bind362
Udgave nummer1
Sider (fra-til)6-9
Antal sider4
ISSN0304-3940
DOI
StatusUdgivet - 13 maj 2004
Udgivet eksterntJa

Fingeraftryk

Dyk ned i forskningsemnerne om 'No effect of MDMA (ecstasy) on cell death and 5-HT2A receptor density in organotypic rat hippocampal cultures'. Sammen danner de et unikt fingeraftryk.

Citationsformater