Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

NF-kappaB signaling mediates vascular smooth muscle endothelin type B receptor expression in resistance arteries

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Drug-drug interactions between vitamin K antagonists and statins: a systematic review

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  2. The Danish model for the quick and safe implementation of infliximab and etanercept biosimilars

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Use of prescription drugs in the older adult population-a nationwide pharmacoepidemiological study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. The effects of 2 weeks of statin treatment on mitochondrial respiratory capacity in middle-aged males: the LIFESTAT study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Hormonal influences in migraine - interactions of oestrogen, oxytocin and CGRP

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  2. CGRP and migraine; from bench to bedside

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer
Vascular smooth muscle cells (SMC) endothelin type B (ET(B)) receptor upregulation results in strong vasoconstriction and reduction of local blood flow. We hypothesizes that the underlying molecular mechanisms involve transcriptional factor nuclear factor-kappaB (NF-kappaB) pathway. ET(B) receptor upregulation and activation of NF-kappaB were studied at functional contraction (in vitro myograph), mRNA (real-time PCR), and protein (Western blot and immunocytochemistry) levels during organ culture of rat mesenteric arteries. Organ culture of the artery segments induced a time-dependent strong contractile response to sarafotoxin 6c in parallel with enhanced expression of ET(B) receptor mRNA and protein in the SMC. Western blot experiments demonstrated that phosphorylation of NF-kappaB p65 was time-dependently induced during organ culture starting at 1h. In addition, cytoplasmic IkB degradation occurred in parallel with nuclear NF-kappaB accumulation following organ culture. The enhanced expression of ET(B) receptor protein was apparent at 3h in the SMC and while enhanced ET(B) receptor-mediated contractions occurred first at 12h. The specific IkappaB inhibitors, IMD-0354 (N-(3,5-Bis-trifluoromethylphenyl)-5-chloro-2-hydroxybenzamide) and Wedelolactone (7-Methoxy-5,11,12-trihydroxycoumestan), abolished the organ culture induced ET(B) receptor upregulation. The intracellular NF-kappaB pathway is involved in the process of induced expression of vascular SMC ET(B) receptors.
OriginalsprogEngelsk
TidsskriftEuropean journal of pharmacology
Vol/bind637
Udgave nummer1-3
Sider (fra-til)148-54
Antal sider7
DOI
StatusUdgivet - 10 jul. 2010

ID: 32311384