Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Analysis of Mass Cytometry Data

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Full-Length Open Reading Frame Amplification of Hepatitis C Virus

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Next Generation Sequencing-Based Fetal ABO Blood Group Prediction by Analysis of Cell-Free DNA from Maternal Plasma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Targeted Rhesus immunoglobulin for RhD negative women undergoing an induced abortion: a clinical pilot study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Blood group genotyping of blood donors: validation of a highly accurate routine method

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Noninvasive fetal RHD genotyping to guide targeted anti-D prophylaxis-an external quality assessment workshop

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

The KEL1 antigen can give rise to immunization of KEL2 mothers. Maternal antibodies can be transferred to the fetus and destroy fetal red blood cells and their stem cell precursors and give rise to serious fetal disease. It is important to be able to predict the fetal KEL status in order to intervene in those pregnancies where the fetus is at risk, and to ascertain when the fetus is not at risk. Technically it can be demanding to predict KEL1 status from a maternal blood sample. The KEL1 allele is based on a single SNP present in about 1-10 % of cell-free maternal DNA after gestation week 10. Here we describe our protocol for antenatal prediction of fetal KEL1 status by NGS analysis of maternal DNA on a MiSeq instrument.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology
Vol/bind1310
Sider (fra-til)115-21
Antal sider7
ISSN1064-3745
DOI
StatusUdgivet - 2015

ID: 45690382