TY - JOUR
T1 - New species in the genus Francisella (Gammaproteobacteria; Francisellaceae); Francisella piscicida sp. nov. isolated from cod (Gadus morhua)
AU - Ottem, Karl F
AU - Nylund, Are
AU - Karlsbakk, Egil
AU - Friis-Møller, Alice
AU - Krossøy, Bjørn
AU - Knappskog, Dag
PY - 2007
Y1 - 2007
N2 - A Francisella strain, GM2212, previously isolated from moribund farmed Atlantic cod (Gadus morhua) in Norway, is closely related to Francisella philomiragia among Francisella spp. according to its complete 16S rDNA, 16S-23S intergenic spacer, 23S rDNA, 23S-5S intergenic spacer, 5S rDNA, FopA, lipoprotein TUL4 (LpnA), malate dehydrogenase and hypothetical lipoprotein (LpnB) sequences. A comparison between GM2212 and the type strain of Francisella philomiragia were performed by DNA-DNA hybridization and fatty acid analysis. The DNA-DNA hybridization showed a 70% similarity. The fatty acid analysis showed only minor differences between the Francisella isolates. Due to the inconclusive result from the DNA-DNA hybridisation, major emphasis concerning the status of this isolate is made on previously published molecular, phenotypic and biochemical characters. All characteristics taken together support the establishment of GM2212 as a novel species, for which the name Francisella piscicida sp. nov. is proposed (=CNCM I-3511(T) = DSM 18777(T) = LMG registration number not yet available).
AB - A Francisella strain, GM2212, previously isolated from moribund farmed Atlantic cod (Gadus morhua) in Norway, is closely related to Francisella philomiragia among Francisella spp. according to its complete 16S rDNA, 16S-23S intergenic spacer, 23S rDNA, 23S-5S intergenic spacer, 5S rDNA, FopA, lipoprotein TUL4 (LpnA), malate dehydrogenase and hypothetical lipoprotein (LpnB) sequences. A comparison between GM2212 and the type strain of Francisella philomiragia were performed by DNA-DNA hybridization and fatty acid analysis. The DNA-DNA hybridization showed a 70% similarity. The fatty acid analysis showed only minor differences between the Francisella isolates. Due to the inconclusive result from the DNA-DNA hybridisation, major emphasis concerning the status of this isolate is made on previously published molecular, phenotypic and biochemical characters. All characteristics taken together support the establishment of GM2212 as a novel species, for which the name Francisella piscicida sp. nov. is proposed (=CNCM I-3511(T) = DSM 18777(T) = LMG registration number not yet available).
KW - Animals
KW - Bacterial Typing Techniques
KW - Fatty Acids
KW - Fish Diseases
KW - Francisella
KW - Gadus morhua
KW - Gram-Negative Bacterial Infections
KW - Nucleic Acid Hybridization
KW - RNA, Ribosomal, 16S
KW - Sequence Analysis, DNA
KW - Species Specificity
U2 - 10.1007/s00203-007-0274-1
DO - 10.1007/s00203-007-0274-1
M3 - Journal article
C2 - 17619856
SN - 0302-8933
VL - 188
SP - 547
EP - 550
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 5
ER -