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Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System

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Harvard

Dmytriyeva, O, de Diego Ajenjo, A, Lundø, K, Hertz, H, Rasmussen, KK, Christiansen, AT, Klingelhofer, J, Nielsen, AL, Hoeber, J, Kozlova, E, Woldbye, DPD & Pankratova, S 2020, 'Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System', ACS Chemical Neuroscience, bind 11, nr. 9, s. 1270-1282. https://doi.org/10.1021/acschemneuro.9b00685

APA

Dmytriyeva, O., de Diego Ajenjo, A., Lundø, K., Hertz, H., Rasmussen, K. K., Christiansen, A. T., Klingelhofer, J., Nielsen, A. L., Hoeber, J., Kozlova, E., Woldbye, D. P. D., & Pankratova, S. (2020). Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System. ACS Chemical Neuroscience, 11(9), 1270-1282. https://doi.org/10.1021/acschemneuro.9b00685

CBE

Dmytriyeva O, de Diego Ajenjo A, Lundø K, Hertz H, Rasmussen KK, Christiansen AT, Klingelhofer J, Nielsen AL, Hoeber J, Kozlova E, Woldbye DPD, Pankratova S. 2020. Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System. ACS Chemical Neuroscience. 11(9):1270-1282. https://doi.org/10.1021/acschemneuro.9b00685

MLA

Vancouver

Author

Dmytriyeva, Oksana ; de Diego Ajenjo, Amaia ; Lundø, Kathrine ; Hertz, Henrik ; Rasmussen, Kim K ; Christiansen, Anders T ; Klingelhofer, Jorg ; Nielsen, Alexander L ; Hoeber, Jan ; Kozlova, Elena ; Woldbye, David P D ; Pankratova, Stanislava. / Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System. I: ACS Chemical Neuroscience. 2020 ; Bind 11, Nr. 9. s. 1270-1282.

Bibtex

@article{f7b9733980034ea9a1db5862eb8b692b,
title = "Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System",
abstract = "Vascular endothelial growth factor B (VEGFB) is a pleiotropic trophic factor, which in contrast to the closely related VEGFA is known to have a limited effect on angiogenesis. VEGFB improves survival in various tissues including the nervous system, where the effect was observed mainly for peripheral neurons. The neurotrophic effect of VEGFB on central nervous system neurons has been less investigated. Here we demonstrated that VEGFB promotes neurite outgrowth from primary cerebellar granule, hippocampal, and retinal neurons in vitro. VEGFB protected hippocampal and retinal neurons from both oxidative stress and glutamate-induced neuronal death. The VEGF receptor 1 (VEGFR1) is required for VEGFB-induced neurotrophic and neuroprotective effects. Using a structure-based approach, we designed short peptides, termed Vefin1-7, mimicking the binding interface of VEGFB to VEGFR1. Vefins were analyzed for their secondary structure and binding to VEGF receptors and compared with previously described peptides derived from VEGFA, another ligand of VEGFR1. We show that Vefins have neurotrophic and neuroprotective effects on primary hippocampal, cerebellar granule, and retinal neurons in vitro with potencies comparable to VEGFB. Similar to VEGFB, Vefins were not mitogenic for MCF-7 cancer cells. Furthermore, one of the peptides, Vefin7, even dose-dependently inhibited the proliferation of MCF-7 cells in vitro. Unraveling the neurotrophic and neuroprotective potentials of VEGFB, the only nonangiogenic factor of the VEGF family, is promising for the development of neuroprotective peptide-based therapies.",
author = "Oksana Dmytriyeva and {de Diego Ajenjo}, Amaia and Kathrine Lund{\o} and Henrik Hertz and Rasmussen, {Kim K} and Christiansen, {Anders T} and Jorg Klingelhofer and Nielsen, {Alexander L} and Jan Hoeber and Elena Kozlova and Woldbye, {David P D} and Stanislava Pankratova",
year = "2020",
month = may,
day = "6",
doi = "10.1021/acschemneuro.9b00685",
language = "English",
volume = "11",
pages = "1270--1282",
journal = "ACS Chemical Neuroscience",
issn = "1948-7193",
publisher = "American Chemical Society",
number = "9",

}

RIS

TY - JOUR

T1 - Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System

AU - Dmytriyeva, Oksana

AU - de Diego Ajenjo, Amaia

AU - Lundø, Kathrine

AU - Hertz, Henrik

AU - Rasmussen, Kim K

AU - Christiansen, Anders T

AU - Klingelhofer, Jorg

AU - Nielsen, Alexander L

AU - Hoeber, Jan

AU - Kozlova, Elena

AU - Woldbye, David P D

AU - Pankratova, Stanislava

PY - 2020/5/6

Y1 - 2020/5/6

N2 - Vascular endothelial growth factor B (VEGFB) is a pleiotropic trophic factor, which in contrast to the closely related VEGFA is known to have a limited effect on angiogenesis. VEGFB improves survival in various tissues including the nervous system, where the effect was observed mainly for peripheral neurons. The neurotrophic effect of VEGFB on central nervous system neurons has been less investigated. Here we demonstrated that VEGFB promotes neurite outgrowth from primary cerebellar granule, hippocampal, and retinal neurons in vitro. VEGFB protected hippocampal and retinal neurons from both oxidative stress and glutamate-induced neuronal death. The VEGF receptor 1 (VEGFR1) is required for VEGFB-induced neurotrophic and neuroprotective effects. Using a structure-based approach, we designed short peptides, termed Vefin1-7, mimicking the binding interface of VEGFB to VEGFR1. Vefins were analyzed for their secondary structure and binding to VEGF receptors and compared with previously described peptides derived from VEGFA, another ligand of VEGFR1. We show that Vefins have neurotrophic and neuroprotective effects on primary hippocampal, cerebellar granule, and retinal neurons in vitro with potencies comparable to VEGFB. Similar to VEGFB, Vefins were not mitogenic for MCF-7 cancer cells. Furthermore, one of the peptides, Vefin7, even dose-dependently inhibited the proliferation of MCF-7 cells in vitro. Unraveling the neurotrophic and neuroprotective potentials of VEGFB, the only nonangiogenic factor of the VEGF family, is promising for the development of neuroprotective peptide-based therapies.

AB - Vascular endothelial growth factor B (VEGFB) is a pleiotropic trophic factor, which in contrast to the closely related VEGFA is known to have a limited effect on angiogenesis. VEGFB improves survival in various tissues including the nervous system, where the effect was observed mainly for peripheral neurons. The neurotrophic effect of VEGFB on central nervous system neurons has been less investigated. Here we demonstrated that VEGFB promotes neurite outgrowth from primary cerebellar granule, hippocampal, and retinal neurons in vitro. VEGFB protected hippocampal and retinal neurons from both oxidative stress and glutamate-induced neuronal death. The VEGF receptor 1 (VEGFR1) is required for VEGFB-induced neurotrophic and neuroprotective effects. Using a structure-based approach, we designed short peptides, termed Vefin1-7, mimicking the binding interface of VEGFB to VEGFR1. Vefins were analyzed for their secondary structure and binding to VEGF receptors and compared with previously described peptides derived from VEGFA, another ligand of VEGFR1. We show that Vefins have neurotrophic and neuroprotective effects on primary hippocampal, cerebellar granule, and retinal neurons in vitro with potencies comparable to VEGFB. Similar to VEGFB, Vefins were not mitogenic for MCF-7 cancer cells. Furthermore, one of the peptides, Vefin7, even dose-dependently inhibited the proliferation of MCF-7 cells in vitro. Unraveling the neurotrophic and neuroprotective potentials of VEGFB, the only nonangiogenic factor of the VEGF family, is promising for the development of neuroprotective peptide-based therapies.

U2 - 10.1021/acschemneuro.9b00685

DO - 10.1021/acschemneuro.9b00685

M3 - Journal article

C2 - 32283014

VL - 11

SP - 1270

EP - 1282

JO - ACS Chemical Neuroscience

JF - ACS Chemical Neuroscience

SN - 1948-7193

IS - 9

ER -

ID: 64730421