Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series

Karin Holst Lauridsen; Kristine Boisen Olsen; Eva Løbner Lund; Tomas O Jensen; Thomas Ingemann Pedersen; Zitta Barrella Harboe; Valeria Antsupova; Lasse Dam Rasmussen; Dennis Röser; Jytte Banner; Kristina Træholt Franck; Veronika Vorobieva Solholm Jensen

doi:10.1177/2632010X221139096

Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series

Karin Holst Lauridsen^*, Kristine Boisen Olsen, Eva Løbner Lund, Tomas O Jensen, Thomas Ingemann Pedersen, Zitta Barrella Harboe, Valeria Antsupova, Lasse Dam Rasmussen, Dennis Röser, Jytte Banner, Kristina Træholt Franck, Veronika Vorobieva Solholm Jensen

^*Corresponding author af dette arbejde

Abstrakt

BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.

METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.

CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.

Originalsprog	Engelsk
Tidsskrift	Clinical pathology (Thousand Oaks, Ventura County, Calif.)
Vol/bind	15
Sider (fra-til)	2632010X221139096
ISSN	2632-010X
DOI	https://doi.org/10.1177/2632010X221139096
Status	Udgivet - dec. 2022

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10.1177/2632010X221139096

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Citationsformater

Lauridsen, K. H., Olsen, K. B., Lund, E. L., Jensen, T. O., Pedersen, T. I., Harboe, Z. B., Antsupova, V., Rasmussen, L. D., Röser, D., Banner, J., Franck, K. T., & Vorobieva Solholm Jensen, V. (2022). Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. Clinical pathology (Thousand Oaks, Ventura County, Calif.), 15, 2632010X221139096. https://doi.org/10.1177/2632010X221139096

Neurological Complications in COVID-19 Patients : Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. / Lauridsen, Karin Holst; Olsen, Kristine Boisen; Lund, Eva Løbner et al.

I: Clinical pathology (Thousand Oaks, Ventura County, Calif.), Bind 15, 12.2022, s. 2632010X221139096.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Lauridsen, KH, Olsen, KB, Lund, EL, Jensen, TO, Pedersen, TI , Harboe, ZB , Antsupova, V, Rasmussen, LD, Röser, D, Banner, J, Franck, KT & Vorobieva Solholm Jensen, V 2022, 'Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series', Clinical pathology (Thousand Oaks, Ventura County, Calif.), bind 15, s. 2632010X221139096. https://doi.org/10.1177/2632010X221139096

Lauridsen KH, Olsen KB, Lund EL, Jensen TO, Pedersen TI , Harboe ZB et al. Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. Clinical pathology (Thousand Oaks, Ventura County, Calif.). 2022 dec.;15:2632010X221139096. https://doi.org/10.1177/2632010X221139096

Lauridsen, Karin Holst ; Olsen, Kristine Boisen ; Lund, Eva Løbner et al. / Neurological Complications in COVID-19 Patients : Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. I: Clinical pathology (Thousand Oaks, Ventura County, Calif.). 2022 ; Bind 15. s. 2632010X221139096.

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title = "Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series",

abstract = "BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.",

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T1 - Neurological Complications in COVID-19 Patients

T2 - Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series

AU - Lauridsen, Karin Holst

AU - Olsen, Kristine Boisen

AU - Lund, Eva Løbner

AU - Jensen, Tomas O

AU - Pedersen, Thomas Ingemann

AU - Harboe, Zitta Barrella

AU - Antsupova, Valeria

AU - Rasmussen, Lasse Dam

AU - Röser, Dennis

AU - Banner, Jytte

AU - Franck, Kristina Træholt

AU - Vorobieva Solholm Jensen, Veronika

PY - 2022/12

Y1 - 2022/12

N2 - BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.

AB - BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.

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M3 - Journal article

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