TY - JOUR
T1 - Negative cooperativity between juxtaposed E-box and cAMP/TPA responsive elements in the cholecystokinin gene promoter
AU - Rourke, I J
AU - Hansen, T V
AU - Nerlov, C
AU - Rehfeld, J F
AU - Nielsen, F C
PY - 1999/4/1
Y1 - 1999/4/1
N2 - The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.
AB - The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.
KW - Animals
KW - Base Sequence
KW - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
KW - Basic-Leucine Zipper Transcription Factors
KW - Cholecystokinin/genetics
KW - Cyclic AMP/metabolism
KW - Cyclic AMP Response Element-Binding Protein/genetics
KW - DNA-Binding Proteins/genetics
KW - Gene Expression Regulation
KW - Helix-Loop-Helix Motifs
KW - Humans
KW - Leucine Zippers
KW - Mice
KW - Molecular Sequence Data
KW - Mutagenesis
KW - Promoter Regions, Genetic
KW - Proto-Oncogene Proteins c-fos/genetics
KW - Proto-Oncogene Proteins c-jun/genetics
KW - Proto-Oncogene Proteins c-myc/genetics
KW - Response Elements
KW - Tetradecanoylphorbol Acetate/metabolism
KW - Transcription Factors/genetics
KW - Tumor Cells, Cultured
U2 - 10.1016/s0014-5793(99)00320-8
DO - 10.1016/s0014-5793(99)00320-8
M3 - Journal article
C2 - 10217400
SN - 0014-5793
VL - 448
SP - 15
EP - 18
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -