TY - JOUR
T1 - Myocardial gene expression of angiogenic factors in human chronic ischemic myocardium
T2 - influence of acute ischemia/cardioplegia and reperfusion
AU - Wang, Yongzhong
AU - Gabrielsen, Anders
AU - Lawler, Patrick R
AU - Paulsson-Berne, Gabrielle
AU - Steinbrüchel, Daniel A
AU - Hansson, Goran K
AU - Kastrup, Jens
PY - 2006
Y1 - 2006
N2 - OBJECTIVE: Angiogenic therapies in animals have demonstrated the development of new blood vessels within ischemic myocardium. However, results from clinical protein and gene angiogenic trials have been less impressive. The present study aimed to investigate the expression of angiogenic genes in human chronic ischemic myocardium and the influence of acute ischemia/cardioplegia and reperfusion on their expression.METHODS: Myocardial biopsies were taken from chronic ischemic and nonischemic myocardium in 15 patients with stable angina pectoris during coronary bypass surgery. Tissue samples were evaluated by oligonucleotide microarray and quantitative real-time PCR for the expression of angiogenic factors.RESULTS: There was identical baseline expression of VEGF-A and VEGF-C mRNA in chronic ischemic myocardium compared with nonischemic myocardium. Reperfusion increased the gene expression of VEGF-A and VEGF-C mRNA both in nonischemic and ischemic myocardium. VEGF-A protein was detected mainly in the extracellular matrix around the cardiomyocytes in ischemic myocardium.CONCLUSION: These data suggest that the nonconclusive VEGF gene therapy trials chronic coronary artery disease was not due to a preexisting upregulation of VEGF in chronic ischemic myocardium. There might be room for further therapeutic angiogenesis in chronic ischemic myocardium.
AB - OBJECTIVE: Angiogenic therapies in animals have demonstrated the development of new blood vessels within ischemic myocardium. However, results from clinical protein and gene angiogenic trials have been less impressive. The present study aimed to investigate the expression of angiogenic genes in human chronic ischemic myocardium and the influence of acute ischemia/cardioplegia and reperfusion on their expression.METHODS: Myocardial biopsies were taken from chronic ischemic and nonischemic myocardium in 15 patients with stable angina pectoris during coronary bypass surgery. Tissue samples were evaluated by oligonucleotide microarray and quantitative real-time PCR for the expression of angiogenic factors.RESULTS: There was identical baseline expression of VEGF-A and VEGF-C mRNA in chronic ischemic myocardium compared with nonischemic myocardium. Reperfusion increased the gene expression of VEGF-A and VEGF-C mRNA both in nonischemic and ischemic myocardium. VEGF-A protein was detected mainly in the extracellular matrix around the cardiomyocytes in ischemic myocardium.CONCLUSION: These data suggest that the nonconclusive VEGF gene therapy trials chronic coronary artery disease was not due to a preexisting upregulation of VEGF in chronic ischemic myocardium. There might be room for further therapeutic angiogenesis in chronic ischemic myocardium.
KW - Aged
KW - Angina Pectoris/pathology
KW - Angiogenic Proteins/genetics
KW - Biopsy
KW - Chronic Disease
KW - Female
KW - Gene Expression Regulation
KW - Heart Arrest, Induced
KW - Humans
KW - Male
KW - Myocardial Ischemia/metabolism
KW - Myocardium/metabolism
KW - RNA, Messenger/analysis
KW - Reperfusion
KW - Vascular Endothelial Growth Factor A/genetics
KW - Vascular Endothelial Growth Factor C/genetics
U2 - 10.1080/10739680600556811
DO - 10.1080/10739680600556811
M3 - Journal article
C2 - 16627361
SN - 1073-9688
VL - 13
SP - 187
EP - 197
JO - Microcirculation (New York, N.Y. : 1994)
JF - Microcirculation (New York, N.Y. : 1994)
IS - 3
ER -