Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Monoclonal antibodies against a synthetic peptide from human immunodeficiency virus type 1 Nef protein.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

  • L Steinaa
  • A M Wulff
  • T Saermark
Vis graf over relationer
Monoclonal antibodies against a synthetic peptide (aa 138-152) from HIV-1 Nef protein were produced and characterized. Three hybridoma lines producing monoclonal antibodies (MAbs) against the synthetic peptide were generated by fusion between P3-X63 Ag8.653 myeloma cells and BALB/c splenocytes from mice immunized with the synthetic peptide coupled to keyhole limpet hemocyanin (KLH). The hybridomas were screened and selected by ELISA with the peptide coupled to bovine serum albumin (BSA) immobilized to the polystyrene surface and specificity for the peptide was confirmed by competitive ELISA with the peptide free in solution. The reactions of the MAbs with a 5-aa motif (WCYKL) included in the sequence were examined with synthetic peptides and two of the MAbs reacted with the motif. The recognitions of recombinant full-length Nef protein were also tested. One MAb reacted with the protein in both ELISA and dot blot, and one only in dot blot, whereas the last MAb did not recognize the recombinant full-length Nef protein.
Bidragets oversatte titelMonoclonal antibodies against a synthetic peptide from human immunodeficiency virus type 1 Nef protein.
OriginalsprogEngelsk
TidsskriftHybridoma
Vol/bind13
Udgave nummer5
Sider (fra-til)383-388
Antal sider6
ISSN0272-457X
StatusUdgivet - 1994

ID: 32516599