Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

miRNA profiles in plasma from patients with sleep disorders reveal dysregulation of miRNAs in narcolepsy and other central hypersomnias

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Functional brown adipose tissue and sympathetic activity after cold exposure in humans with type 1 narcolepsy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Cerebrospinal fluid biomarkers of neurodegeneration are decreased or normal in narcolepsy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Breathing Disturbances Without Hypoxia Are Associated With Objective Sleepiness in Sleep Apnea

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Onset of Impaired Sleep and Cardiovascular Disease Risk Factors: A Longitudinal Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. HLA DQB1*06:02 negative narcolepsy with hypocretin/orexin deficiency

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Altered surface expression of P2Y11 receptor with narcolepsy-associated mutations

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Sleep in cluster headache revisited: Results from a controlled actigraphic study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

STUDY OBJECTIVES: MicroRNAs (miRNAs) have been implicated in the pathogenesis of human diseases including neurological disorders. The aim is to address the involvement of miRNAs in the pathophysiology of central hypersomnias including autoimmune narcolepsy with cataplexy and hypocretin deficiency (type 1 narcolepsy), narcolepsy without cataplexy (type 2 narcolepsy), and idiopathic hypersomnia.

DESIGN: We conducted high-throughput analysis of miRNA in plasma from three groups of patients-with type 1 narcolepsy, type 2 narcolepsy, and idiopathic hypersomnia, respectively-in comparison with healthy controls using quantitative real-time polymerase chain reaction (qPCR) panels.

SETTING: University hospital based sleep clinic and research laboratories.

PATIENTS: Twelve patients with type 1 narcolepsy, 12 patients with type 2 narcolepsy, 12 patients with idiopathic hypersomnia, and 12 healthy controls.

MEASUREMENTS AND RESULTS: By analyzing miRNA in plasma with qPCR we identified 50, 24, and 6 miRNAs that were different in patients with type 1 narcolepsy, type 2 narcolepsy, and idiopathic hypersomnia, respectively, compared with healthy controls. Twenty miRNA candidates who fulfilled the criteria of at least two-fold difference and p-value < 0.05 were selected to validate the miRNA changes in an independent cohort of patients. Four miRNAs differed significantly between type 1 narcolepsy patients and healthy controls. Levels of miR-30c, let-7f, and miR-26a were higher, whereas the level of miR-130a was lower in type 1 narcolepsy than healthy controls. The miRNA differences were not specific for type 1 narcolepsy, since the levels of the four miRNAs were also altered in patients with type 2 narcolepsy and idiopathic hypersomnia compared with healthy controls.

CONCLUSION: The levels of four miRNAs differed in plasma from patients with type 1 narcolepsy, type 2 narcolepsy and idiopathic hypersomnia suggesting that alterations of miRNAs may be involved in the pathophysiology of central hypersomnias.

OriginalsprogEngelsk
TidsskriftSleep
Vol/bind37
Udgave nummer9
Sider (fra-til)1525-33
Antal sider9
ISSN0161-8105
DOI
StatusUdgivet - sep. 2014

ID: 44992083