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MicroRNA expression analysis and Multiplex ligation-dependent probe amplification in metastatic and non-metastatic uveal melanoma

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@article{21f2654566494c839500fdd0529e646d,
title = "MicroRNA expression analysis and Multiplex ligation-dependent probe amplification in metastatic and non-metastatic uveal melanoma",
abstract = "PURPOSE: To determine the association of microRNA expression and chromosomal changes with metastasis and survival in uveal melanoma (UM).METHODS: Thirty-six patients with UM were selected based on the metastatic status, and clinicopathological data were collected. Multiplex ligation-dependent probe amplification (MLPA) was used to identify chromosomal changes. Chromosomal changes and clinicopathological data were correlated with survival and metastasis. The microRNA expression was analysed in 26 of the 36 archived UM samples. Unsupervised analysis, differential expression analysis and Cox regression analysis were performed to determine the association with metastasis and survival.RESULTS: Metastasis and metastatic death occurred in 20 patients, two patients died of other causes and one patient of unknown causes. A significant association between increasing size category (p = 0.002, log-rank), extraocular extension (p = 0.001), chromosome 3 loss (p = 0.033) and 1p loss (p = 0.030) and development of metastases was observed. Tumour, node, metastasis (TNM) staging showed a significant association with survival (p < 0.0001, log-rank). Adjusting for gender and age TNM size category T4 (p = 0.016, Cox regression analysis), mixed (p = 0.029) and epithelioid (p = 0.0058) cell types, chromosome 3 loss (p = 0.014) and 8q gain (p = 0.010) were significant prognosticators for a poor survival. Hierarchical clustering divided the UM into three groups based on microRNA expression. The clusters showed no association with clinical or histopathological features, TNM staging, metastasis or survival. Differential expression analysis did not reveal microRNAs related to metastasis or survival.CONCLUSIONS: The prognostic significance of chromosome 3 loss and 8q gain identified by MLPA analysis was confirmed in archived UM samples. The value of microRNA expression as a predictor of metastasis and survival in UM could not be confirmed.",
keywords = "Adult, Aged, Aged, 80 and over, Chromosome Aberrations, Chromosomes, Human, Pair 3, Chromosomes, Human, Pair 8, Female, Gene Expression Profiling, Humans, Lymphatic Metastasis, Male, Melanoma, MicroRNAs, Middle Aged, Multiplex Polymerase Chain Reaction, Neoplasm Proteins, Proportional Hazards Models, Survival Rate, Uveal Neoplasms",
author = "Ann-Cathrine Larsen and Line Holst and Bogumil Kaczkowski and Andersen, {Morten T} and Valentina Manf{\'e} and Siersma, {Volkert D} and Miriam Kolko and Kiilgaard, {Jens F} and Ole Winther and Prause, {Jan U} and Robert Gniadecki and Steffen Heegaard",
note = "{\textcopyright} 2013 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.",
year = "2014",
month = sep,
doi = "10.1111/aos.12322",
language = "English",
volume = "92",
pages = "541--9",
journal = "Acta Ophthalmologica (Online)",
issn = "1755-3768",
publisher = "Wiley-Blackwell Publishing, Inc",
number = "6",

}

RIS

TY - JOUR

T1 - MicroRNA expression analysis and Multiplex ligation-dependent probe amplification in metastatic and non-metastatic uveal melanoma

AU - Larsen, Ann-Cathrine

AU - Holst, Line

AU - Kaczkowski, Bogumil

AU - Andersen, Morten T

AU - Manfé, Valentina

AU - Siersma, Volkert D

AU - Kolko, Miriam

AU - Kiilgaard, Jens F

AU - Winther, Ole

AU - Prause, Jan U

AU - Gniadecki, Robert

AU - Heegaard, Steffen

N1 - © 2013 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

PY - 2014/9

Y1 - 2014/9

N2 - PURPOSE: To determine the association of microRNA expression and chromosomal changes with metastasis and survival in uveal melanoma (UM).METHODS: Thirty-six patients with UM were selected based on the metastatic status, and clinicopathological data were collected. Multiplex ligation-dependent probe amplification (MLPA) was used to identify chromosomal changes. Chromosomal changes and clinicopathological data were correlated with survival and metastasis. The microRNA expression was analysed in 26 of the 36 archived UM samples. Unsupervised analysis, differential expression analysis and Cox regression analysis were performed to determine the association with metastasis and survival.RESULTS: Metastasis and metastatic death occurred in 20 patients, two patients died of other causes and one patient of unknown causes. A significant association between increasing size category (p = 0.002, log-rank), extraocular extension (p = 0.001), chromosome 3 loss (p = 0.033) and 1p loss (p = 0.030) and development of metastases was observed. Tumour, node, metastasis (TNM) staging showed a significant association with survival (p < 0.0001, log-rank). Adjusting for gender and age TNM size category T4 (p = 0.016, Cox regression analysis), mixed (p = 0.029) and epithelioid (p = 0.0058) cell types, chromosome 3 loss (p = 0.014) and 8q gain (p = 0.010) were significant prognosticators for a poor survival. Hierarchical clustering divided the UM into three groups based on microRNA expression. The clusters showed no association with clinical or histopathological features, TNM staging, metastasis or survival. Differential expression analysis did not reveal microRNAs related to metastasis or survival.CONCLUSIONS: The prognostic significance of chromosome 3 loss and 8q gain identified by MLPA analysis was confirmed in archived UM samples. The value of microRNA expression as a predictor of metastasis and survival in UM could not be confirmed.

AB - PURPOSE: To determine the association of microRNA expression and chromosomal changes with metastasis and survival in uveal melanoma (UM).METHODS: Thirty-six patients with UM were selected based on the metastatic status, and clinicopathological data were collected. Multiplex ligation-dependent probe amplification (MLPA) was used to identify chromosomal changes. Chromosomal changes and clinicopathological data were correlated with survival and metastasis. The microRNA expression was analysed in 26 of the 36 archived UM samples. Unsupervised analysis, differential expression analysis and Cox regression analysis were performed to determine the association with metastasis and survival.RESULTS: Metastasis and metastatic death occurred in 20 patients, two patients died of other causes and one patient of unknown causes. A significant association between increasing size category (p = 0.002, log-rank), extraocular extension (p = 0.001), chromosome 3 loss (p = 0.033) and 1p loss (p = 0.030) and development of metastases was observed. Tumour, node, metastasis (TNM) staging showed a significant association with survival (p < 0.0001, log-rank). Adjusting for gender and age TNM size category T4 (p = 0.016, Cox regression analysis), mixed (p = 0.029) and epithelioid (p = 0.0058) cell types, chromosome 3 loss (p = 0.014) and 8q gain (p = 0.010) were significant prognosticators for a poor survival. Hierarchical clustering divided the UM into three groups based on microRNA expression. The clusters showed no association with clinical or histopathological features, TNM staging, metastasis or survival. Differential expression analysis did not reveal microRNAs related to metastasis or survival.CONCLUSIONS: The prognostic significance of chromosome 3 loss and 8q gain identified by MLPA analysis was confirmed in archived UM samples. The value of microRNA expression as a predictor of metastasis and survival in UM could not be confirmed.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Chromosome Aberrations

KW - Chromosomes, Human, Pair 3

KW - Chromosomes, Human, Pair 8

KW - Female

KW - Gene Expression Profiling

KW - Humans

KW - Lymphatic Metastasis

KW - Male

KW - Melanoma

KW - MicroRNAs

KW - Middle Aged

KW - Multiplex Polymerase Chain Reaction

KW - Neoplasm Proteins

KW - Proportional Hazards Models

KW - Survival Rate

KW - Uveal Neoplasms

U2 - 10.1111/aos.12322

DO - 10.1111/aos.12322

M3 - Journal article

C2 - 24373459

VL - 92

SP - 541

EP - 549

JO - Acta Ophthalmologica (Online)

JF - Acta Ophthalmologica (Online)

SN - 1755-3768

IS - 6

ER -

ID: 45004733