Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
E-pub ahead of print

Microcrack surface density in the human otic capsule: An unbiased stereological quantification

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Bone signaling in middle ear development: a genome-wide differential expression analysis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The viability of perilabyrinthine osteocytes: a quantitative study using bulk-stained undecalcified human temporal bones

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. The three-dimensional arrangement of the myocytes aggregated together within the mammalian ventricular myocardium

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Macrophage-like cells in the muscularis externa of mouse small intestine

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Plexus muscularis profundus and associated interstitial cells. II. Ultrastructural studies of mouse small intestine

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Validating the 7-item Eustachian Tube Dysfunction Questionnaire in Danish

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The Effect of Simulator-Integrated Tutoring for Guidance in Virtual Reality Simulation Training

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Stereologic Investigation of Mastoid Air Cell Geometry: Volume, Surface Area, and Anisotropy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Bone is continuously remodeled to repair and strengthen degenerative bone with accumulating dead osteocytes and microfractures. Inner ear osteoprotegerin (OPG)-mediated inhibition of otic capsular bone remodeling causes excessive perilabyrinthine bone degeneration. Consequently, microcracks accumulate around the inner ear. Microcracks cause osteocyte apoptosis and may disrupt the canalicular network connecting osteocytes. Despite their linear microscopic appearance, microcracks are three-dimensional disruption planes and represent surface areas inside a tissue space. With an elevated microcrack burden the number of disconnected osteocytes is expected to increase. This may prove relevant to ongoing research in otic focal pathologies like otosclerosis. Therefore, an unbiased quantification of the microcrack surface density (mm2 /mm3 ) in the human otic capsule is essential. In this study unbiased stereology was applied to undecalcified bulk stained human temporal bones to demonstrate its feasibility in describing the three-dimensional reality behind two dimensional observations of microcracks. A total of 28 human temporal bones and five ribs were bulk stained in basic fuchsin, serially sectioned and hand-ground to a thickness of 80-120 μm. Both horizontal and vertical sections were produced and compared. This study showed that surface density of microcracks was significantly higher around the inner ear compared to ribs. Furthermore, no significant difference in microcrack surface density between horizontal and vertical sections in the temporal bone was demonstrated.

OriginalsprogEngelsk
TidsskriftAnatomical record (Hoboken, N.J. : 2007)
ISSN1932-8486
DOI
StatusE-pub ahead of print - 11 okt. 2020

Bibliografisk note

© 2020 American Association for Anatomy.

ID: 61231258