Micro-ELISA for the quantitation of human urinary and serum retinol-binding protein

T Jensen, M Deckert, A Dawnay, B Feldt-Rasmussen

    17 Citationer (Scopus)

    Abstract

    A highly sensitive enzyme linked immunoadsorbent assay for determining retinol-binding protein in urine and serum is described. Commercially available reagents are used. The standard curve ranges from 1.2-24 micrograms/l, recovery of retinol-binding protein added to urine was 99-102% (n = 10) and dilution of urine was linear. The within-assay coefficient of variation ranged from 1.2-3.1% and the day-to-day coefficient of variation from 9.2-10.5% depending on concentration. The correlation with urinary retinol-binding protein determined by radioimmunoassay was good (n = 90, r = 0.95). In vitro experiments show that retinol-binding protein is stable in urine with pH 5.2.

    OriginalsprogEngelsk
    TidsskriftDiabetes Research: Clinical & Experimental
    Vol/bind10
    Udgave nummer2
    Sider (fra-til)93-5
    Antal sider3
    ISSN0265-5985
    StatusUdgivet - feb. 1989

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