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Michaëlis--Menten kinetics of phenazone elimination in the perfused pig liver

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@article{2135f20e83fe45ad86bac146a43c0775,
title = "Micha{\"e}lis--Menten kinetics of phenazone elimination in the perfused pig liver",
abstract = "The purpose of the present study was to define the elimination kinetics of phenazone (NFN) in the isolated perfused pig liver. In five experiments phenazone was administered as constant infusion to obtain steady-state periods over a wide range of concentrations. The elimination of phenazone followed saturation kinetics (concentrations 0.1-12 mmol x 1(-1) and the maximal elimination rate (Vmax) was on average 102 mumol x min-1 x kg-1 liver and the Micha{\"e}lis-constant (Km) of 2.6 mmol x 1(-1). Estimates of Vmax and Km for the microsomal phenazone hydroxylase activity measured in liver biopsies found to be considerably lower than in the perfused liver. The hepatic elimination of phenazone during perfusion of pig liver at phenazone concentrations corresponding to human therapeutic doses follows first-order kinetics.",
keywords = "Animals, Antipyrine, Biotransformation, Cytochrome P-450 Enzyme System, Female, Liver, Liver Circulation, Microsomes, Liver, Organ Size, Perfusion, Swine",
author = "B Andreasen and K Tonnesen and A Rabol and Susanne Keiding",
year = "1977",
month = jan,
language = "English",
volume = "40",
pages = "1--13",
journal = "Acta Pharmacologica et Toxicologica",
issn = "0001-6683",
publisher = "Munksgaard International Publishers",
number = "1",

}

RIS

TY - JOUR

T1 - Michaëlis--Menten kinetics of phenazone elimination in the perfused pig liver

AU - Andreasen, B

AU - Tonnesen, K

AU - Rabol, A

AU - Keiding, Susanne

PY - 1977/1

Y1 - 1977/1

N2 - The purpose of the present study was to define the elimination kinetics of phenazone (NFN) in the isolated perfused pig liver. In five experiments phenazone was administered as constant infusion to obtain steady-state periods over a wide range of concentrations. The elimination of phenazone followed saturation kinetics (concentrations 0.1-12 mmol x 1(-1) and the maximal elimination rate (Vmax) was on average 102 mumol x min-1 x kg-1 liver and the Michaëlis-constant (Km) of 2.6 mmol x 1(-1). Estimates of Vmax and Km for the microsomal phenazone hydroxylase activity measured in liver biopsies found to be considerably lower than in the perfused liver. The hepatic elimination of phenazone during perfusion of pig liver at phenazone concentrations corresponding to human therapeutic doses follows first-order kinetics.

AB - The purpose of the present study was to define the elimination kinetics of phenazone (NFN) in the isolated perfused pig liver. In five experiments phenazone was administered as constant infusion to obtain steady-state periods over a wide range of concentrations. The elimination of phenazone followed saturation kinetics (concentrations 0.1-12 mmol x 1(-1) and the maximal elimination rate (Vmax) was on average 102 mumol x min-1 x kg-1 liver and the Michaëlis-constant (Km) of 2.6 mmol x 1(-1). Estimates of Vmax and Km for the microsomal phenazone hydroxylase activity measured in liver biopsies found to be considerably lower than in the perfused liver. The hepatic elimination of phenazone during perfusion of pig liver at phenazone concentrations corresponding to human therapeutic doses follows first-order kinetics.

KW - Animals

KW - Antipyrine

KW - Biotransformation

KW - Cytochrome P-450 Enzyme System

KW - Female

KW - Liver

KW - Liver Circulation

KW - Microsomes, Liver

KW - Organ Size

KW - Perfusion

KW - Swine

M3 - Journal article

C2 - 576352

VL - 40

SP - 1

EP - 13

JO - Acta Pharmacologica et Toxicologica

JF - Acta Pharmacologica et Toxicologica

SN - 0001-6683

IS - 1

ER -

ID: 39050783