Methodological approaches to antigen identification in soluble immune complexes; A model study

S Husby; S E Svehag; H Nielsen; N Høiby; P O Schiøtz

doi:10.1111/j.1699-0463.1981.tb02679.x

Methodological approaches to antigen identification in soluble immune complexes; A model study

S Husby
, S E Svehag
, H Nielsen
, N Høiby
, P O Schiøtz

3 Citationer (Scopus)

Abstract

The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.

Originalsprog	Engelsk
Tidsskrift	Acta pathologica et microbiologica Scandinavica. Section C, Immunology
Vol/bind	89
Udgave nummer	2
Sider (fra-til)	155-60
Antal sider	6
ISSN	0304-1328
DOI	https://doi.org/10.1111/j.1699-0463.1981.tb02679.x
Status	Udgivet - apr. 1981
Udgivet eksternt	Ja

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10.1111/j.1699-0463.1981.tb02679.x

Citationsformater

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title = "Methodological approaches to antigen identification in soluble immune complexes; A model study",

abstract = "The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.",

keywords = "Adsorption, Antigen-Antibody Complex/analysis, Antigens, Bacterial/analysis, Epitopes/analysis, Humans, Immunoelectrophoresis, Immunologic Techniques, Radioimmunoassay",

author = "S Husby and Svehag, \{S E\} and H Nielsen and N H{\o}iby and Schi{\o}tz, \{P O\}",

year = "1981",

month = apr,

doi = "10.1111/j.1699-0463.1981.tb02679.x",

language = "English",

volume = "89",

pages = "155--60",

journal = "Acta pathologica et microbiologica Scandinavica. Section C, Immunology",

issn = "0304-1328",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "2",

}

TY - JOUR

T1 - Methodological approaches to antigen identification in soluble immune complexes; A model study

AU - Husby, S

AU - Svehag, S E

AU - Nielsen, H

AU - Høiby, N

AU - Schiøtz, P O

PY - 1981/4

Y1 - 1981/4

N2 - The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.

AB - The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.

KW - Adsorption

KW - Antigen-Antibody Complex/analysis

KW - Antigens, Bacterial/analysis

KW - Epitopes/analysis

KW - Humans

KW - Immunoelectrophoresis

KW - Immunologic Techniques

KW - Radioimmunoassay

U2 - 10.1111/j.1699-0463.1981.tb02679.x

DO - 10.1111/j.1699-0463.1981.tb02679.x

M3 - Journal article

C2 - 6170207

SN - 0304-1328

VL - 89

SP - 155

EP - 160

JO - Acta pathologica et microbiologica Scandinavica. Section C, Immunology

JF - Acta pathologica et microbiologica Scandinavica. Section C, Immunology

IS - 2

ER -

Methodological approaches to antigen identification in soluble immune complexes; A model study

Abstract

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